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pSL5200 (pDonor_ISGst3)

Plasmid

#210875

Purpose

Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

ISGst3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTopo_Ex2Donor_pPGK-PURO

Plasmid

#198862

Purpose

Donor template for CRISPR/Cas9 targeting of TERT Ex2

Depositor

Agnel Sfeir

Article

Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

Insert

PURO

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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DUX4 pDonor221

Plasmid

#187801

Purpose

DUX4 entry clone

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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LMNA_mScarlet-I_Donor

Plasmid

#178092

Purpose

HDR donor plasmid to tag LMNA with mScarlet-I using eSpCas9(1.1)_No_FLAG_LMNA_G2.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

LMNA-mScarlet-I HDR donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pDonor-GDH

Plasmid

#140629

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

Depositor

Sheng Yang

Article

Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

Insert

Mini-Tn6677, T7lac-GDH gene cassette

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I_Donor

Plasmid

#173207

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV_Actb HR donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97309

Purpose

HR donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb HR donor

Use

AAV and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSL0715 (pDonor_R1-57)

Plasmid

#130644

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened right end. Total transposon size = 910 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short R)

Use

CRISPR; Transposon

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR-SNCAe3-WT

Plasmid

#85847

Purpose

Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

SNCA exon 3 homology arms (SNCA Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pDonorTp-fugfp

Plasmid

#211790

Purpose

pDonorTp with addition of fugfp gene from pUS252 outside of the FRT sites

Depositor

Silvia Cardona

Insert

fuGFP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[GFP]

Plasmid

#213903

Purpose

Mariner transposon expressing mNeonGreen with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#173208

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I-TOSI donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[RFP]

Plasmid

#213904

Purpose

Mariner transposon expressing mCherry with cam selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with cam resistance and mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2

Plasmid

#213907

Purpose

Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn-pOUT

Plasmid

#213905

Purpose

Mariner transposon with outward-facing pJ23104 promoter

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and outward-facine pJ23104 promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_Tn[sfGFP]

Plasmid

#213902

Purpose

Mariner transposon expressing sfGFP with kan selection casette

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and superfolder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2_pJEx

Plasmid

#213908

Purpose

Barcoded mariner transposon with crystal violet-inducible outward promoter - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and EilR/pJEx and FseI-SbfI sites for barcode insertion

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Tags

Expression

Yeast

Mutation

NA

Promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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