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hTph2p-Luc

Plasmid

#223526

Purpose

Firefly-luciferase reporter expression driven by human Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Tph2 promoter (TPH2 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

hTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-Luc

Plasmid

#223663

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut1-Luc

Plasmid

#223664

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 1

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut2-Luc

Plasmid

#223665

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at RRE site 2

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut3-Luc

Plasmid

#223666

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 3

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-mut4-Luc

Plasmid

#223667

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter point mutation at PRE site 4

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Promoter

mTph2 promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1b-3X FLAG

Plasmid

#218536

Purpose

human FLVCR1b with C-terminal 3X_FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Alternative isoform

Promoter

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

Plasmid

#218534

Purpose

human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgPet-1-hSyn-mCherry-KASH

Plasmid

#223227

Purpose

guideRNA targeting the mouse Pet-1 (Fev)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Fev (Fev Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET_His6-SUMO-TEV-TtCsm6

Plasmid

#172486

Purpose

Bacterial expression of codon-optimized His-SUMO-TtCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

T. thermophilus Csm6 (codon optimized) (csm6 Synthetic)

Use

Tags

His-SUMO-TEV

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Use

Tags

His6 and bdSUMO

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a_His-TEV-EiCsm6

Plasmid

#172487

Purpose

Bacterial expression of codon-optimized His-EiCsm6 with a TEV protease cleavage site.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

Enterococcus italicus Csm6 (codon optimized)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7 promoter and lac operator

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Dox-SOScat

Plasmid

#207329

Purpose

PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized catalytic domain of SOS (SOScat)

Depositor

Jared Toettcher

Article

Underhill et al Development. 2023 Aug 15;150(16):dev2016

Insert

son of sevenless homolog 2 variant, catalytic domain

Use

Tags

myristoylation tag - BFP

Expression

Bacterial

Mutation

range: 593 to 1078

Promoter

Tet Response Element

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gLacZ.Cas9-T2A-GFP

Plasmid

#170361

Purpose

Negative control plasmid used for Crispr/cas9 based disruption.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLV.U6.gDNMT1.Cas9-T2A-GFP

Plasmid

#170362

Purpose

Plasmid used for Crispr/cas9 based disruption of human DNMT1.

Depositor

Johan Jakobsson

Article

Jonsson et al Nat Commun. 2019 Jul 18;10(1):3182. doi:

Insert

Cas9-T2A-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.flex.H2B.GFP

Plasmid

#170368

Purpose

Cre-inducible AAV vector expressing nuclear GFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

flex H2B GFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSyn.Cre

Plasmid

#170367

Purpose

AAV vector expressing Cre under human synapsin promotor

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.flex.H2B.RFP

Plasmid

#170373

Purpose

Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

Cre inducible H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Tags

Expression

Mutation

Promoter

human Synapsin

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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