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Plasmid#206410PurposeMammalian expression of hARFGAP2DepositorInsertARFGAP2 (ARFGAP2 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ku70-TC
Plasmid#46955PurposePlasmid containing the targeting construct to insert a GFP-FLAG tag at the N-terminus of the human XRCC6 gene coding for Ku70.DepositorInsertXRCC6 targeting construct (XRCC6 Human)
UseAAV; RTagsGFP-FLAGExpressionMutationPromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRCaMP1
Plasmid#125245PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRCaMP1
UseTagsExpressionMammalianMutationW44YPromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-NEW-fRGECO2
Plasmid#125248PurposeExpresses red fluorescent calcium sensor in mammalian cellsDepositorInsertfRGECO2
UseTagsNES signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
UseTagsCitrine YFPExpressionPlantMutationPromoter1.5 kb MUM4 promoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
UseTagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
UseTagsmRFPExpressionPlantMutationPromoterRD21A nativeAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRGECO1
Plasmid#125243PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRGECO1
UseTagsExpressionMammalianMutationD323APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30b GluBP600n
Plasmid#119835PurposeProduction of recombinant GluBP600n high affinity protein in E. coliDepositorInsertGluBP600n
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlul
Plasmid#119832PurposeProduction of recombinant iGluSnFR T92A low affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlul
UseTagsGSTExpressionBacterialMutationT92APromoterT7Available sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102 ΔAmpH Δ2x+ve
Plasmid#129484PurposeExpression of Glo3 ΔAmpH lacking the COPI-binding motif in E.ColiDepositorInsertADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
gMUM2-Citrine
Plasmid#181965PurposeMUM2 protein fused to Citrine, under control of native promoterDepositorInsertMUM2 (MUM2 Mustard Weed)
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM2Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MUM2SP-Citrine
Plasmid#181963PurposeMUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertMUM2 signal peptide, fused to the Citrine YFP in pAD
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM4pro 1.5kb fragmentAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR207-MUM2sp-PelA
Plasmid#170722PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme PelA (AN2331.2)DepositorInsertPectin lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglB
Plasmid#170721PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglB (AN6395.2)DepositorInsertRhamnogalacturonan lyase B
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR207-MUM2sp-RglA
Plasmid#170720PurposeGateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the RG-I degrading enzyme RglA (AN7135.2)DepositorInsertRhamnogalacturonan lyase A
UseGateway donor vector / entry cloneTagsExpressionMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p415 Glo3 R59K Δ2x+ve
Plasmid#112655PurposeExpression S.cerevisiaeDepositorInsertGlo3 R59K Δ2x+ve
UseTagsExpressionYeastMutationR59K, mutations (R223E, K224E, K225E, K233E, K234…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415 GFP-Glo3 Δ2x+ve
Plasmid#112662PurposeExpression S.cerevisiaeDepositorInsertGFP- Glo3 Δ2x+ve
UseTagssfGFPExpressionYeastMutationMutations (R223E, K224E, K225E, K233E, K234E, K23…PromoterMet25Available sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluSnFR E25R
Plasmid#119831PurposeProduction of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluSnFR E25R
UseTagsGSTExpressionBacterialMutationE25RPromoterT7Available sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGlum
Plasmid#119829PurposeProduction of recombinant iGluSnFR R24K medium affinity variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGlum
UseTagsGSTExpressionBacterialMutationR24KPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only