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Plasmid#17157DepositorInsertsiamois promoter (sia1.L Frog)
UseLuciferaseTagsluciferaseExpressionMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Phoenix dactylifera
Plasmid#78117PurposeNt His-tag Phoenix dactylifera Sacsin 1-1295DepositorInsertSacsin Phoenix dactylifera 1-1295
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDK4-luciferase active (880)
Plasmid#86657PurposeLuciferase reporter containing CDK4 promoterDepositorInsertCDK4 promoter (CDK4 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutation867 base pairs including up to coding nucleotide …PromoterCDK5Available sinceJune 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Nelumbo nucifera
Plasmid#75358PurposeNt His-tag Nelumbo nucifera Sacsin 1-1313DepositorInsertSacsin Nelumbo nucifera 1-1313
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-FERM10
Plasmid#59126PurposeFAK fret control sensor without kinase domain with 10 nm linkerDepositorInsertFocal Adhesion Kinase
UseTagsFLAG, mCerulean, and mCitrineExpressionMammalianMutationPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
GL4.10 Prg4 promoter-luciferase
Plasmid#195034PurposePrg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vectorDepositorInsertPrg4 promoter (PRG4 Bovine)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-MYO10-FERM-ITGBD
Plasmid#194855PurposeExpress EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.DepositorInsertMYO10 S2001_F2002insA/T2009D (MYO10 Human)
UseTagsEGFPExpressionMammalianMutationMYO10 has the following mutations S2001_F2002insA…PromoterCMVAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Plekhh1-MyTH-FERM
Plasmid#187370PurposeExpresses human Plekhh1-MyTH-FERM labelled with EGFPDepositorInsertPlekhh1-MyTH-FERM
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Nelumbo nucifera
Plasmid#75326PurposeGST fusion Nelumbo nucifera Sacsin 1-1313DepositorInsertSacsin Nelumbo nucifera 1-1313
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-SIRPT1-Phoenix dactylifera
Plasmid#75328PurposeGST fusion Phoenix dactylifera Sacsin 1-1295DepositorInsertSacsin Phoenix dactylifera 1-1295
UseTagsGST-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorInsertPGC-1 alpha promoter (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_empty
Plasmid#99297PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_neg.cont
Plasmid#99315PurposeNegative control for luciferase validation vectorsDepositorInsertneutral region; chr3: 55198740-55200041
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_SV40
Plasmid#99309PurposeLuciferase validation vector with SV40 enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertSV40 enhancer
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_ORI_CMV
Plasmid#99312PurposeLuciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activityDepositorInsertCMV enhancer
UseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI stuffer
Plasmid#50920PurposeU6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sitesDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRM210 LGP (Luciferase GFP Puro)
Plasmid#174722PurposeExpresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance geneDepositorInsertsFirefly Luciferase
eGFP
pac
UseLentiviral and LuciferaseTagsE2A linker and F2A linkerExpressionMammalianMutationPromoterMND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
STARR-seq luciferase validation vector_mP_empty
Plasmid#99298PurposeLuciferase validation vector; empty vector without insert to determine baselineDepositorTypeEmpty backboneUseLuciferase; Starr-seq luciferase validation vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR
Plasmid#182249PurposeHuman HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of HNRNPAB gene
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only