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Plasmid#199768PurposeExpresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1D1
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry2-sgRNA (empty)
Plasmid#198330PurposeCustomisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporterDepositorInsertU6-sgRNA(F+E) empty
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH45
Plasmid#199767PurposeExpresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH45
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Blast NT shRNA
Plasmid#123306PurposeExpression of a non-targeting shRNADepositorInsertNon-targeting shRNA
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAPa-3xFLAG-pCDNA
Plasmid#201963PurposeMammalian expression of Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP26
Plasmid#199769PurposeExpresses split-intein fused MSP26 for circularization during expression (internal His-Tag)DepositorInsertcMSP26
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH4-6
Plasmid#199766PurposeExpresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH46
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WF
Plasmid#108337PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BR
Plasmid#108336PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WF
Plasmid#108333PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)BF
Plasmid#108334PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(+)WR
Plasmid#108335PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BF
Plasmid#108338PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)WR
Plasmid#108339PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pML2(−)BR
Plasmid#108340PurposeMIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF-DEST51 LacZ
Plasmid#123292PurposeMammalian expression of control truncated LacZDepositorInsertLacZ
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRAPa(R150A)-3xFLAG-pCDNA
Plasmid#201966PurposeMammalian expression of mutated Translocon-associated protein subunit alphaDepositorInsertSSR1 (SSR1 Human)
UseTags3xFLAG-tagExpressionMammalianMutationR150APromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP3
Plasmid#42310DepositorInsertVAMP 3 (VAMP3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hPMCA4b
Plasmid#47589DepositorInsertPMCA4x/b (ATP2B4 Human)
UseTagsEGFPExpressionMammalianMutationK442R and P673L relative to NP_001675.3PromoterCMVAvailable sinceAug. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti c-MYC-DDK-Puro GFP
Plasmid#123299PurposeMammalian expression of GFP C-MYC-FLAGDepositorInsertGFP
UseLentiviralTagsMYC-FLAGExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only