Skip to main content
Addgene
Showing: 241 - 260 of 753 results

  5. pAPV-FLAG-DsRed2-ER-5

    Plasmid
    #170620
    Purpose
    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-FLAG-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170620-plasmid-map-sequence-id-335780

    Enlarge

  7. pAPV-mEmerald-DsRed2-ER-5

    Plasmid
    #170681
    Purpose
    Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-mEmerald-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170681-plasmid-map-sequence-id-335805

    Enlarge

  8. pAPV-DsRed2-mEmerald-ER-5

    Plasmid
    #170715
    Purpose
    Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-mEmerald-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170715-plasmid-map-sequence-id-335807

    Enlarge

  10. pISV-DsRed1-nopP

    Plasmid
    #171025
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.
    Depositor
    Christian Staehelin
    Insert
    nopP
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    2 X EN 35S
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171025-plasmid-map-sequence-id-337715

    Enlarge

  12. pUPD DsRed-CT (GB1183)

    Plasmid
    #160556
    Purpose
    DsRed for C-terminal fusions
    Depositor
    Diego Orzaez
    Insert
    DsRed_CT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Dec. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    160556-plasmid-map-sequence-id-319285

    Enlarge

  13. pHD-dsRed-attP_dCrk_flankingHRs

    Plasmid
    #131141
    Purpose
    Contains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).
    Depositor
    Mark Peifer
    Article
    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.
    Inserts
    Crk Upstream Homology Region (Crk Fly)
    Crk Downstream Homology Region (Crk Fly)
    Use
    CRISPR and Cre/Lox; Homology directed repair temp…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    131141-plasmid-map-sequence-id-258858

    Enlarge

  14. AV5-siRNA-dsRed

    Plasmid
    #124973
    Purpose
    siRNA
    Depositor
    Kazuhiro Oka
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    124973-plasmid-map-sequence-id-243953

    Enlarge

  16. DsRed-Nix-S212A

    Plasmid
    #100762
    Purpose
    Mammalian expression of Fluorescent Nix S212A
    Depositor
    Joseph Gordon
    Insert
    BCL2 interacting protein 3 like (BNIP3L Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    S212A
    Promoter
    CMV
    Available since
    Nov. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    100762-plasmid-map-sequence-id-193062

    Enlarge

  17. DsRed-CPI-17-wt-NLS

    Plasmid
    #100788
    Purpose
    Mammalian expression of Fluorescent CPI-17 nuclear
    Depositor
    Joseph Gordon
    Insert
    CPI-17-NLS (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100788-plasmid-map-sequence-id-193218

    Enlarge

  18. DsRed-CPI-17-wt-NES

    Plasmid
    #100787
    Purpose
    Mammalian expression of Fluorescent CPI-17 cytosolic
    Depositor
    Joseph Gordon
    Insert
    CPI-17-NES (PPP1R14A Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    100787-plasmid-map-sequence-id-193216

    Enlarge

  19. DsRed-Nix-S212D

    Plasmid
    #100764
    Purpose
    Mammalian expression of Fluorescent Nix S212D
    Depositor
    Joseph Gordon
    Insert
    dsRed-Nix-S212D (BNIP3L Human)
    Use
    Tags
    DsRed
    Expression
    Mammalian
    Mutation
    S212D
    Promoter
    CMV
    Available since
    Sept. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    100764-plasmid-map-sequence-id-193067

    Enlarge

Showing: 241 - 260 of 753 results