Showing: 241 - 252 of 252 results
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Plasmid#101837Purposedrives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locusDepositorInsertkaiBC S431A T432A with p0050 promoter
UseCyanobacteria cloning vectorTagsExpressionMutationS431A T432APromoterAvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaTagsExpressionMutationPromoterKanRAvailabilityAcademic Institutions and Nonprofits only -
pRL2697
Plasmid#70693PurposepDU1-E. coli shuttle vector (Cm-r Em-r) with extended polylinkerDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRL2960
Plasmid#70694PurposepDU1-E. coli shuttle vector (Km-r) with extended polylinkerDepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pRL1342
Plasmid#70691PurposeRSF1010-based vector with extensive polylinker, and resistance to Cm and Em. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.DepositorTypeEmpty backboneUseBacterial shuttle vectorTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
gCOTS-pyl
Plasmid#92048PurposeThis is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.DepositorInsertsPyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseCyanobacteria s. elongatus genome recombination i…TagsExpressionMutationPromoterLeuP (native S. elongatus promoter) and PrcbLAvailabilityAcademic Institutions and Nonprofits only -
pAM5187
Plasmid#120086PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid.DepositorInsertpAM4787 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAM4787
Plasmid#120088PurposeCyanobacteria synthetic plasmid to test the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS).DepositorInsertPlasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
UseOtherTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAM4788
Plasmid#120089PurposeCyanobacteria synthetic plasmid harboring the minimal replicon of Synechococcus elongatus PCC 7942 small plasmid (pANS) to test the pSYSA-TA1 toxin–antitoxin cassette.DepositorInsertpAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
UseOtherTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAM5188
Plasmid#120087PurposeCyanobacteria cloning vector based on Synechococcus elongatus PCC 7942 small plasmid, harboring a toxin-antitoxin cassette to improve the plasmid stability without antibiotic selection.DepositorInsertpAM4788 where the promoter/reporter device (PconII-LTRBS-yemGFP) was replaced with a MCS from the pBluescript vector (pBS MCS).
UseOtherTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagExpressionMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …AvailabilityAcademic Institutions and Nonprofits only -
pRL838
Plasmid#70680PurposeThis vector was used to generate a library of ca. 17-kb sequences of clones as part of a genomic sequence strategy and were then used to complement mutations.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 241 - 252 of 252 results