Addgene

We narrowed to 11,633 results for: CAR

Showing: 241 - 260 of 11633 results

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pmScarlet-H_NES_C1

Plasmid

#85061

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Use

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-I-SYFP2

Plasmid

#189761

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-I-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mCardinal-Calnexin-N-14

Plasmid

#56154

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Calnexin (CANX Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCAR-OF reporter

Plasmid

#16627

Depositor

Bert Vogelstein

Article

Nicolaides et al Mol Cell Biol. 1998 Mar . 18(3):1635-41.

Insert

CAR-OF

Use

Tags

beta-galactosidase

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
cARA12-mRFP

Plasmid

#181961

Purpose

ARA12 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ARA12 (ARA12 Mustard Weed)

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

TBA2

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1Q90A

Plasmid

#133249

Purpose

Express FLAG-tagged MCART1Q90A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, Q9…

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1R182A

Plasmid

#133252

Purpose

Express FLAG-tagged MCART1R182A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

MCART1 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, R1…

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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p3XFlag-CARMIL2

Plasmid

#118740

Purpose

To express CARMIL2 with Flag tag on N-terminus

Depositor

John Cooper

Article

Lanier et al Mol Biol Cell. 2015 Dec 15;26(25):4577-8

Insert

CARMIL2 (CARMIL2 Human)

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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mCardinal-Rab4a-7

Plasmid

#56174

Purpose

Localization: Ras GTPase, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Rab4a (RAB4A Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mCardinal-Fibrillarin-7

Plasmid

#56160

Purpose

Localization: Nuclear - Nucleolus, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Fibrillarin (FBL Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#178087

Purpose

HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

mScarlet-I mTOR signaling indicator (TOSI)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-BRG1_Neo_FKBPF36V_mScarlet

Plasmid

#163875

Purpose

Acute Depletion of BRG1-mScarlet

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCardinal-Vimentin-7

Plasmid

#56178

Purpose

Localization: Intermediate Filaments, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Vimentin (VIM Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CDS12_mScarletI-linker

Plasmid

#136088

Purpose

FP for N-term fusion with a CTAG without linker

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS12_mScarletI(noStop)-SerGly10xAlaSer

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI/ SapI domesticated

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-MannII-N-10

Plasmid

#56167

Purpose

Localization: Mannosidase/Golgi, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

MannII

Use

Tags

mCardinal

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits
peGFPC2-CARKL

Plasmid

#51748

Purpose

expression of eGFP-fused mouse sedoheptulokinase (CARKL, SHPK)

Depositor

Markus Ralser

Article

Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

Insert

SHPK (Shpk Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-MCART1W283A

Plasmid

#138409

Purpose

Express FLAG-tagged MCART1W283A in mammalian cells

Depositor

David Sabatini

Article

Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

Insert

SLC25A51 (SLC25A51 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

codon-optimized for expression in human cells, W2…

Promoter

Available since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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