Addgene

Showing: 241 - 260 of 4106 results

Results per page:

Sort results by:

pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

UBC

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-hPGK_NKX2-1

Plasmid

#221494

Purpose

NKX2-1 short isoform lentiviral overexpression using hPGK promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-SFFV_NKX2-1

Plasmid

#221496

Purpose

NKX2-1 short isoform lentiviral overexpression using SFFV promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SF-1 (antisense)

Plasmid

#28181

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Reverse Orientation

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
SF-1-A172S

Plasmid

#28185

Depositor

Bernard Schimmer

Article

Frigeri et al Endocrinology. 2002 Oct . 143(10):4031-7

Insert

SF-1 (Nr5a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

A172S (polymorphism)

Promoter

Available since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
Lsg-LRRK2-1

Plasmid

#199277

Purpose

nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 nick

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGen2.1-redCALWY-1

Plasmid

#50739

Purpose

Expresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.

Depositor

Maarten Merkx

Article

Lindenburg et al ACS Chem Biol. 2013 Oct 18;8(10):2133-9.

Insert

gene for redCALWY-1 Zn2+ sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 27, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BufAI-1

Plasmid

#128650

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Hygromycin-TTF-1

Plasmid

#119173

Purpose

Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Zeo-TTF-1

Plasmid

#75089

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

TTF-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Promoter

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
CPSF4-1

Plasmid

#155478

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF4 (CPSF4 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DDX27-1

Plasmid

#155514

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX27 (DDX27 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-Yap1-E1-#1

Plasmid

#171517

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330A-Yap1-E2-#1

Plasmid

#171518

Purpose

deletion of a genomic locus in Yap1 gene

Depositor

Hans Schöler

Article

Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

Insert

Yap1 (Yap1 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2-dADAM17 (#1)

Plasmid

#177259

Purpose

A knockout vector for the dog Adam17

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

A gRNA targeting the dog Adam17 gene and the cDNA of CRISPR-Cas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX4T-1 Srap1

Plasmid

#89691

Purpose

Expresses murine Srap1 in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Murine SRAP domain ortholog Srap1 (Hmces Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
hVoS 2.0 thy-1

Plasmid

#45282

Depositor

Meyer B. Jackson

Article

Wang et al Biophys J. 2010 Oct 6;99(7):2355-65. doi

Insert

CeFP

Use

Targeting

Tags

GAP43 motif and truncated h-ras motif

Expression

Mutation

Promoter

thy1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only
hVoS 1.5 thy-1

Plasmid

#45281

Depositor

Meyer B. Jackson

Article

Wang et al Biophys J. 2010 Oct 6;99(7):2355-65. doi

Insert

CeFP

Use

Targeting

Tags

truncated h-ras motif

Expression

Mutation

Promoter

thy1

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pLKO.1-Teton-puro-shFDPS #1

Plasmid

#198759

Purpose

conditional knockdown of FDPS

Depositor

Hyonchol Jang

Article

Kim et al Exp Mol Med. 2018 Oct 17;50(10):137. doi

Insert

shFDPS #1 (FDPS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-sg-1

Plasmid

#190607

Purpose

Expresses a gRNA for base editing the EGFP Kozak sequence of pWPT-/mEGFP-1T-IRES-mCherry

Depositor

Alessandro Quattrone

Article

Ambrosini et al Nucleic Acids Res. 2022 Oct 14;50(18):10

Insert

gRNA sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge