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Plasmid#32827DepositorInsertAMOT p130 (AMOT Human)
UseTags2xHAExpressionMammalianMutationE5D, G858D, deletion of amino acids 410-751PromoterCMVAvailable sinceJan. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-sfGFP-DocI
Plasmid#58708PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertsfGFP-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV500 A-SREBP1
Plasmid#33357DepositorInsertSREBP1 (Srebf1 Cricetulus griseus (hamster))
UseTagsFLAGExpressionMammalianMutationDominant Negative (see notes)PromoterCMVAvailable sinceDec. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCR8-Magneto2.0
Plasmid#74333PurposeFor gateway cloning Magneto2.0 into destination vectorDepositorInsertMagneto2.0
UseGateway vectorTagsFlagExpressionMutationTRPV4: delta 760-871PromoternoneAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMH4
Plasmid#52529Purposeserves as PCR-template to generate HR donors for C-terminal 2xFLAG-tagDepositorInsert2x FLAG-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AILR-LplA-ER
Plasmid#42574DepositorInsertLplA(AILR) with ER-retention sequence KDEL
UseTagsFLAGExpressionMutationCompared to wild-type LplA: tryptophan 37 to alan…PromoterCAGAvailable sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-G
Plasmid#40027DepositorInsertsGFP exon 1
GFP exon 2
beta-globin intron
UseTagsExpressionMammalianMutationinsertion of a loxP site, nucleotides 1-274, and …PromoterCAG (chicken beta globin promoter and CMV enhance…Available sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSF4 CMV intron renilla TRICK CTE polyA
Plasmid#84443PurposeTRICK reporter mRNADepositorInsertRenill-TRICK
UseLuciferase; FrtTagsExpressionMammalianMutationPromoterTet-CMVAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSCT-GAL93-PSPC1
Plasmid#46321DepositorInsertPSPC1 (PSPC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-kanMX6
Plasmid#39293DepositorInsertKanR
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-myc-Rbx1
Plasmid#29506DepositorInsertRbx1 (Rbx1 Mouse)
UseTagsHis and mycExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-His3MX6
Plasmid#41604DepositorInsertHis3
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutationPromoterAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT340_pattB-pCA-GFP-pA-FRT5-pPGK-Flpo-pA
Plasmid#52549Purposetransgenic construct for expression of GFP and FlpoDepositorInsertsPhiC31o attB (full length)
GFP
Flpo recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120391PurposepAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoterDepositorTypeEmpty backboneUseAAVTagsmCherry.3XFLAGExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti GFP-AMOT p130 Y242/287A
Plasmid#32829DepositorInsertAMOT p130 (AMOT Human)
UseLentiviralTagsGFP-AMOTExpressionMammalianMutationY242/287APromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-T-intron-G
Plasmid#36887DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GFP
Plasmid#41615DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGFP(S65T), TRP1 reverse orientation, and yeast AD…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron-T
Plasmid#36885DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIW1
Plasmid#52530Purposeserves as PCR-template to generate HR donors for C-terminal Twin-Strep tagDepositorInsertTwin-STREP-tag
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJP102
Plasmid#176480PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and NatR under control of the Capsaspora actin promoterDepositorInsertsactin > NatR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP103
Plasmid#176481PurposeExpresses mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertsactin > HygR
EF1 > mScarlet
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCA-ATG-intron-tdT3Myc
Plasmid#36883DepositorInserttdTomato-3Myc
UseTags3 Myc tagsExpressionMammalianMutationbeta-globin intron containing a loxP site inserte…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR'-ROMK1-CITE-GFP
Plasmid#21322DepositorInsertROMK1 (Kcnj1 Rat)
UseLentiviralTagsGFP (bicistronic with CITE/IRES)ExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S61A
Plasmid#33087DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS61APromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
MTK2_024
Plasmid#123716PurposeEncodes crippled mouse pPGK1 as a Type 2 part to be used in the MTK systemDepositorInsertpPGK1c
UseTagsExpressionMammalianMutationCrippled Kozac sequencePromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZF-BCB-GCN
Plasmid#127476Purposecoding sequence for synthetic zinc finger ZF-BCB-GCNDepositorInsertZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRB20
Plasmid#52559Purposeserves as PCR-template to generate HR donors for C-terminal YFP-tagDepositorInserteYFP and Blasticigin resistance cassette
UseBlunt cloning kitTagsExpressionMutationPromoterAvailable sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-SETin-FLAG-HA
Plasmid#24999DepositorInsertSET (SET Human)
UseTagsFLAG and HAExpressionMammalianMutationsilent mutations introduced to prevent siRNA targ…PromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYP-SELF
Plasmid#158770PurposeExpression of synaptophysin 1 variant with self-cleaving c-terminal tail.DepositorInsertSynaptophysin 1-NS3/4a (Syt1 Mouse)
UseLentiviralTagsExpressionMutationFlexible linkers, ADVVCC/QMSY cleavage sitePromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Hoxc4
Plasmid#45550DepositorInsertHoxc4 (Hoxc4 Mouse)
UseTagsExpressionMutationPromoterCMV / b-actinAvailable sinceAug. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-CBM-CohI
Plasmid#58709PurposeProbing molecule to perform single molecule force spectroscopy experimentsDepositorInsertCBM-CohI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationchanged Alanine 2 to Cysteine in CBM domainPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZF-ADD-GCN
Plasmid#127475Purposecoding sequence for synthetic zinc finger ZF-ADD-GCNDepositorInsertZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
UseCell-free transcription–translationTagsGCN4 leucine zipperExpressionMutationPromoterT7Available sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT241_(pCA-ATG-intron-tTA2)
Plasmid#36875DepositorInserttTA2
UseTagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-S381A
Plasmid#33090DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationS381APromoterCMVAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-GST
Plasmid#41612DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsGST, TRP1 reverse orientation, and yeast ADH1 ter…ExpressionMutationPromoterAvailable sinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pClneoFLAG mouse RASSF2
Plasmid#37017DepositorInsertRASSF2 (Rassf2 Mouse)
UseTagsFLAGExpressionMammalianMutationD72G and S127FPromoterCMVAvailable sinceAug. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKF251
Plasmid#52532PurposeDouble-GFP reporter for DNA cleavageDepositorInsertDouble-GFP
UseTagsExpressionInsectMutationPromoterubiquitinAvailable sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Flag-YAP-H122L
Plasmid#33086DepositorInsertYAP (YAP1 )
UseTagsFlagExpressionMammalianMutationH122LPromoterCMVAvailable sinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT234_(pCA-G-intron-tTA2)
Plasmid#36874DepositorInsertsGFP
tTA2
beta-globin intron
UseTagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
CAP-AAV.FUS.3
Plasmid#222333PurposeAAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.DepositorInsertAAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
UseAAVTagsExpressionMutationPromoterp41Available sinceAug. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1
Plasmid#209321PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1 expression.DepositorInsertAPOBEC1-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C+F-ArchT-GFP
Plasmid#209700PurposeExpresses ArchT-GFP dependent on the combined activity of CRE- and FLP-recombinasesDepositorInsertArchT-GFP
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D-HA
Plasmid#209234PurposeMammalian expression of ATP10DDepositorInsertATP10D (ATP10D Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/TARG1
Plasmid#172580PurposeFor generation of FlpIn lines with untagged TARG1DepositorInsertTARG1 (OARD1 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR/zeo-TARG1
Plasmid#172574PurposeFor gateway cloning of N-terminal tagged TARG1 constructs.DepositorInsertTARG1 (OARD1 Human)
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK2_025
Plasmid#123717PurposeEncodes crippled human pEF1alpha as a Type 2 part to be used in the MTK systemDepositorInsertpEF1ac
UseTagsExpressionMammalianMutationCrippled Kozac sequencePromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-HipO
Plasmid#128133PurposeThe cell-free adapted backbone, pBEAST, expressing HipO (the enzyme converting hippurate to benzoate) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertHipO
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23101Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only