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4xHRE-MinTK-CRE-ODD

Plasmid

#141147

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE-ODD

Use

Lentiviral

Tags

Expression

Mutation

contains an altered Cre gene modified by the addi…

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pDisplay-mgSrtA

Plasmid

#125792

Purpose

Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.eGFP.2A.P301L-Tau

Plasmid

#140425

Purpose

AAV.CBA.eGFP.2A.P301L-Tau propagation reporter P301L-2N4R-hTau

Depositor

Bradley Hyman

Article

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau(P301L) (MAPT Synthetic, Human)

Use

AAV

Tags

eGFP

Expression

Mutation

changed Proline 301 to Leucine

Promoter

CBA

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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ANO6-Plvx-mCherry-c1

Plasmid

#62554

Purpose

Full length mouse ANO6 tagged with mCherry on the C-terminus

Depositor

Renzhi Han

Article

Zhao et al PLoS One. 2014 Mar 24;9(3):e92749. doi:

Insert

mouse ANO6 (Ano6 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mutation

Promoter

CMV

Available since

March 3, 2015

Availability

Academic Institutions and Nonprofits only

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8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHluorin

Plasmid

#70113

Purpose

Expression of lyso-pHluorin in mammalian cells for optical readout of lysosomal acidification

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHluorin (Cd63 Aequorea victoria, Mouse)

Use

Tags

HA-Tag and pHluorin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PEW-pEF1A-inactEGFP

Plasmid

#212009

Purpose

Cleavable backbone for constitutive expression of TCR alpha and beta chains through lentiviral transduction

Depositor

Catherine Wu

Article

Afeyan et al Nat Protoc. 2024 Nov 8. doi: 10.1038/s41

Insert

Inactivated eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pEF1a

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4-AARE-luc2P-Hygro

Plasmid

#101787

Purpose

Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

3xAARE

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

minimal TATA-box promoter with low basal activity

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX iLOV

Plasmid

#26587

Depositor

John Christie

Article

Chapman et al Proc Natl Acad Sci U S A. 2008 Dec 16. 1

Insert

iLOV

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pHR_SFFv_4D5-WT-Highest-CAR_RHL004

Plasmid

#164826

Purpose

Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

Depositor

Wendell Lim

Article

Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

Insert

AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ADRP

Plasmid

#87161

Purpose

Fluorescent protein localized to lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

ADRP (PLIN2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFB1_hMLH1

Plasmid

#129426

Purpose

Expression human MLH1 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MLH1 (MLH1 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA5a-sufABCDSE

Plasmid

#195056

Purpose

Expresses SUF operon from E. coli from IPTG-inducible promoter

Depositor

Greg Bokinsky

Article

D'Angelo et al Elife. 2022 Mar 4;11:e70936. doi: 10.755

Insert

sufABCDSE

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1_hPMS2

Plasmid

#129425

Purpose

Expression human PMS2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human PMS2 (PMS2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin promoter

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_SFFV

Plasmid

#79121

Purpose

Lentiviral vector for constitutive expression of genes (SFFV promoter)

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Promoter

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p4455 FLAG-hPLIC-2

Plasmid

#8661

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-2 (UBQLN2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pCS2-Arl13b-mCardinal

Plasmid

#194542

Purpose

Expresses Arl13b with mCardinal in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Arl13b (Arl13b Mouse)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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