Addgene

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pGTag-eGFP-CAAX-B-actin

Plasmid

#117816

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

eGFP

Use

Synthetic Biology

Tags

CAAX

Expression

Mutation

Promoter

Available since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-eGFP

Plasmid

#117776

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-mRFP

Plasmid

#117775

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-gcry-eGFP

Plasmid

#117773

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pPRISM-Stop-cmlc2-TagBFP

Plasmid

#117774

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Article

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

3x3 Stop sequence

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pT22Ubm5En2SRE4PCh(#302)

Plasmid

#184089

Purpose

cyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2(SR)-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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Tgfb-Induced-Enhancer:Beta-globin-minimal-promoter-EGFP:pA pDestTol2pA2

Plasmid

#220508

Purpose

TGFb-inducible fluorescent reporter (TIE:EGFP)

Depositor

Leonard Zon

Inserts

TGFb inducible enhancer
beta-globin minimal promoter
SV40 PolyA signal

Use

Zebrafish expression (tol2)

Tags

EGFP

Expression

Mutation

Promoter

Available since

June 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKozak_Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199496

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199482

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199481

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mKate2

Plasmid

#199483

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199486

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_1_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199485

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mTagBFP2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_T2A-Gal4vp16_synCoTC_4xnrUAS-mTagBFP2

Plasmid

#199484

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

Gal4vp16/4xnrUAS-mKate2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKozak_mTagBFP2-T2A-sGFP11x7_synCoTC

Plasmid

#199495

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_0_mTagBFP2-T2A-sGFP11x7_synCoTC

Plasmid

#199478

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC

Plasmid

#199479

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC

Plasmid

#199480

Purpose

Site directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTC

Depositor

Robert Bryson-Richardson

Article

Miles et al bioRxiv 2023

Insert

mTagBFP2-T2A-sGFP11x7

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8CeGFP

Plasmid

#34952

Depositor

Amro Hamdoun

Article

Gokirmak et al J Biol Chem. 2012 Nov 2.

Type

Empty backbone

Use

Sea urchin, zebrafish, xenopus

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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