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Plasmid#176714PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania africana (sender part).DepositorArticleInsertmfalpha1Ka
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK021
Plasmid#176725PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kazachstania africana (receiver part).DepositorArticleInsertste2Ka
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK023
Plasmid#176727PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Kazachstania naganishii (receiver part).DepositorArticleInsertste2Kn
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK033
Plasmid#176737PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Kazachstania naganishii (barrier part).DepositorArticleInsertbar1Kn
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYCTK012
Plasmid#176716PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Kazachstania naganishii (sender part).DepositorArticleInsertmfalpha1Kn
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-03
Plasmid#166927PurposeSTAR-AD1.A6 part (C)DepositorInsertStar Antisense AD1.A6 - Block C - Flanked by BsaI sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC-02
Plasmid#166926PurposeSTAR-AD1.A5 part (C)DepositorInsertStar Antisense AD1.A5- Block C- Flanked by BsaI restriction sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK-GW
Plasmid#126576PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-URA3
Plasmid#122890PurposeLYS2-URA3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPMS008 (JBEI-11596)
Plasmid#87957PurposejStack yeast assembly into plant vectors, Level 1 Intermediary Vector, ColE1originDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR292
Plasmid#79548PurposeOutput plasmid: contains 16-state register that regulates expression of GFP. Implements RSM with 3-input passcode behavior.DepositorInsertmTagBFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR166
Plasmid#79547PurposeOutput plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.DepositorInsertGFPmut3b
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Stmn2
Plasmid#74397PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Stmn2. Not for gene expression.DepositorInsertstathmin-like 2 (Stmn2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2229
Plasmid#67537PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJexpress414_OmpA171_R4cons_Y129H
Plasmid#64302PurposeConservative version of OmpA Redesign 4 with Y129H mutationDepositorInsertOmpA_R4cons_Y129H
UseSynthetic BiologyTags6His and FlAsHExpressionBacterialMutation25 mutations to lipid-facing surfacePromoterT7Available sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-MET17
Plasmid#122885PurposeADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-LEU2
Plasmid#122888PurposeLYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HIS3
Plasmid#122891PurposeTRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-MET17
Plasmid#122893PurposeTRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE5
Plasmid#90339PurposeSTAT3 - Stat3 homodimer gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterSTAT3Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only