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Showing: 2561 - 2580 of 47555 results
  1. pBB328

    Plasmid
    #221533
    Purpose
    Plasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.
    Depositor
    Inserts
    vioABCE
    Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
    Tetracycline antibiotic cassette from pRK415
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    None, contains RBS site
    Available since
    Aug. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. RIX-F2Oct4F3-GFP

    Plasmid
    #216848
    Purpose
    This plasmid is for the production of lentiviral vectors that can be used to track pluripotent stem cells, such as ES cells, iPS and tissue-resident pluripotent cells. GFP expression is lost upon diff
    Depositor
    Insert
    GFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Oct4
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. SOX2-3'gRNA

    Plasmid
    #215543
    Purpose
    gRNA targeting SOX2 3' terminal for CRISPR-Cas9-mediated knock-in
    Depositor
    Insert
    SOX2 (SOX2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. LRP1A-c004

    Plasmid
    #205112
    Purpose
    LRP1 protein expression
    Depositor
    Insert
    LRP1 (LRP1 Human)
    Use
    Tags
    AviTag and GP64sp-6His-TEV
    Expression
    Insect
    Mutation
    WT
    Promoter
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. mCerulean3-Bik-s2193

    Plasmid
    #177425
    Purpose
    To express mCerulean3 fused to the N-terminus of the Bcl-2 family protein, Bik, in a plasmid with Blasticidin resistance. Used to make stable BMK-DKO cell lines.
    Depositor
    Insert
    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    Use
    Tags
    mCerulean3
    Expression
    Mammalian
    Mutation
    Promoter
    Human ferritin
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pDEST27-GST-hSFRP1(aa181-314)

    Plasmid
    #189548
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Netrin (NTR) domain--amino acids 181-314.
    Depositor
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa181-314
    Promoter
    Available since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pDEST27-GST-hSFRP1(aa53-169)

    Plasmid
    #189545
    Purpose
    Protein expression of SFRP1 in mammalian cells. Contains only Frizzled cysteine rich domain (CRD)--amino acids 53-169.
    Depositor
    Insert
    truncated secreted frizzled related protein 1 (SFRP1 Human)
    Use
    Tags
    GST
    Expression
    Mammalian
    Mutation
    contains aa53-169
    Promoter
    Available since
    Oct. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pT22iD36CriT(#388)

    Plasmid
    #184062
    Purpose
    cyclofen-inducible CRE activation in zebrafish permanent transgenic
    Depositor
    Insert
    CRE-ERT2
    Use
    Zebrafish transgenesis (blue eyes)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Dio3
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. GLuc VLP

    Plasmid
    #182213
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).
    Depositor
    Inserts
    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GAL1 and GAL10
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
Showing: 2561 - 2580 of 47555 results