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Plasmid#193246PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Tsc1 geneDepositorInsertsgTsc1#1 (Tsc1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgZfp536#2/Cre
Plasmid#193249PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Zfp536 geneDepositorInsertsgZfp536#2 (Zfp536 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRunx1t1#1/Cre
Plasmid#193235PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Runx1t1 geneDepositorInsertsgRunx1t1#1 (Runx1t1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch3#1/Cre
Plasmid#193227PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch3 geneDepositorInsertsgNotch3#1 (Notch3 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-sgRNA-MS2-U6
Plasmid#171694PurposeTo construct expression vector for tBE-V5-mA3DepositorInsertsgRNA scaffold-MS2-U6
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGG442
Plasmid#165616PurposeVector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)DepositorInsertTEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
UseCRISPRTags3x HA, SV40 NLS, Zif268, and c-Myc-like NLSExpressionYeastMutationCorrected homology relative to wild type SpCas9 (…PromoterTEF1Available sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
EMX1*_sgRNA
Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2_LSEC:Cas9green; erbb2_gRNA171
Plasmid#199338PurposepDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNADepositorInsertsLSEC
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p246 eSpCas9_2gRNAs_mH11
Plasmid#164854PurposegRNA vector for targeting mouse H11 locusDepositorInsertgRNAs for targeting mouse H11 locus
UseTagsExpressionMammalianMutationPromoterU6Available sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol2_claudink:Cas9green; erbb2_gRNA171
Plasmid#199339PurposepDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNADepositorInsertsclaudink
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
zCREST3:Cas9green; EGF_gRNA
Plasmid#199333PurposepDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNADepositorInsertszCREST3
Cas9
nrg1 EGF sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2_NBT:Cas9green; erbb2_gRNA171
Plasmid#199337PurposepDEL134; transgenic construct to express cell-specific Cas9 in neurons; ubiquitous erbb2 sgRNADepositorInsertsNBT
Cas9
erbb2 sgRNA
UseTol2 destination vectorTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR104
Plasmid#187243PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVi U6_gRNA CMV_SadCas9-KRAB
Plasmid#214609PurposeAll-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassetteDepositorInsertsgRNA
SadCas9
UseAAVTagsNP NLS and SV40 NLSExpressionMammalianMutationD10A, N580APromoterCMV and U6Available sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJR103
Plasmid#187242PurposeLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBh-NLS_dCas13d_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191797Purposevector for encoding a human codon-optimized dead RfxCas13d (dCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13d
UseCRISPRTagsExpressionMammalianMutationR239A, H244A, R858A, H863APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMCSG7-dNmeCas9
Plasmid#71475Purposebacterial pMCSG7 expression vector expressing catalytically inactive Nme cas9 with T7 promoter, N-terminal His tag and TEV siteDepositorInsertpMCSG7-Inactive-NmeCas9
UseTags6HisExpressionBacterialMutationD16A H588APromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFTK056
Plasmid#171328PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertSpCas9-NLS
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK063
Plasmid#171335PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m4) (for fusion)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK062
Plasmid#171334PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertdSpCas9(m2) (for fusion)
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationn/aPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only