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Plasmid#180641PurposeBacterial expression for CHMP4B C-terminal tail, residues 156-224. Used for FP competition experiments. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-140DepositorInsertCHMP4B (CHMP4B Human)
UseTagsHIS-SUMOExpressionBacterialMutationResidues 156-224PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169_X
Plasmid#147983PurposeInsect Expression of DmNot1_1-1717-del1156-1169DepositorInsertDmNot1_1-1717-del1156-1169 (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-R1197AN1203AF1204A_X
Plasmid#147984PurposeInsect Expression of DmNot1_1-1717-del1156-1169-R1197AN1203AF1204ADepositorInsertDmNot1_1-1717-del1156-1169-R1197AN1203AF1204A (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA_X
Plasmid#147985PurposeInsect Expression of DmNot1_1-1717-del1156-1169-RNFKGQtoAAASEADepositorInsertDmNot1_1-1717-del1156-1169-RNFKGQtoAAASEA (Not1 Fly)
UseTagsExpressionInsectMutation6 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres_X
Plasmid#147990PurposeInsect Expression of DmNot1_1-1945-K1537SG1562EQ1658A-dsRNAresDepositorInsertDmNot1_1-1945-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation8 silent mutations and two non silent N605S, K759…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-dsRNAres_X
Plasmid#147991PurposeInsect Expression of DmNot1-del1156-1169-dsRNAresDepositorInsertDmNot1-del1156-1169-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147875PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot1-del1156-1169-RNFtoAAA-dsRNAres_W
Plasmid#147859PurposeInsect Expression of DmNot1-del1156-1169-RNFtoAAA-dsRNAresDepositorInsertDmNot1-del1156-1169-RNFtoAAA-dsRNAres (Not1 Fly)
UseTagsExpressionInsectMutation10 silent mutations and two non silent N605S, K75…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsGIGYF2_1-718-del280-310_AI
Plasmid#148949PurposeMammalian Expression of HsGIGYF2_1-718-del280-310DepositorInsertHsGIGYF2_1-718-del280-310 (GIGYF2 Human)
UseTagsExpressionMammalianMutationone silent mutation and one deletion of Q1211 com…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-L640AV646A_AD
Plasmid#148520PurposeMammalian Expression of HseIF4G1-L640AV646ADepositorInsertHseIF4G1-L640AV646A (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-LIVVtoAAAA_AD
Plasmid#148521PurposeMammalian Expression of HseIF4G1-LIVVtoAAAADepositorInsertHseIF4G1-LIVVtoAAAA (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HseIF4G1-Y619AL624AL625A_AD
Plasmid#148522PurposeMammalian Expression of HseIF4G1-Y619AL624AL625ADepositorInsertHseIF4G1-Y619AL624AL625A (EIF4G1 Human)
UseTagsExpressionMammalianMutationone non silent mutation M432V (natural variant is…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110_X
Plasmid#147998PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110DepositorInsertHsNot1iso1_840-1605-del1097-1110 (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA_X
Plasmid#147999PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAADepositorInsertHsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1318-1605-KGRQtoSYAA_W
Plasmid#147896PurposeMammalian Expression of HsNot1iso1_1318-1605-KGRQ-SYAADepositorInsertHsNot1iso1_1318-1605-KGRQ-SYAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W
Plasmid#147881PurposeMammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAresDepositorInsertHsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1-del1097-1110-shRNAres_W
Plasmid#147882PurposeMammalian Expression of HsNot1iso1-del1097-1110-shRNAresDepositorInsertHsNot1iso1-del1097-1110-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres_W
Plasmid#147888PurposeMammalian Expression of HsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1605-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres_W
Plasmid#147890PurposeMammalian Expression of HsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAresDepositorInsertHsNot1iso1_1-1840-IKGRQtoDSYAA-shRNAres (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X
Plasmid#148000PurposeMammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAADepositorInsertHsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)
UseTagsExpressionMammalianMutation1 silent mutation compared to the sequence of iso…PromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only