-
Plasmid#180211PurposeExpresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeastDepositorInsertAs-NF-kB3
UseTagsGAL4 (aa 1-147) fusion proteinExpressionYeastMutationaa 2-412PromoterAdhIAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-As-NF-kB3
Plasmid#180202PurposeExpresses FLAG-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cellsDepositorInsertAs-NF-kB3
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-412PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB-Cterm
Plasmid#180199PurposeExpresses FLAG-tagged Capsaspora NF-kB C-terminal sequences in mammalian cellsDepositorInsertCo-NF-kBCterm
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 542-1224PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
Plasmid#175568PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6DepositorInsertGOXL6
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt
Plasmid#175567PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-5DepositorInsertGOXL5
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL4-Citrine:NOSt
Plasmid#175566PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-4DepositorInsertGOXL4
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL3-Citrine:NOSt
Plasmid#175565PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-3DepositorInsertGOXL3
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt
Plasmid#175564PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2DepositorInsertGOXL2
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM L368G
Plasmid#169985PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (L368G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, L368GPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM R394E
Plasmid#169987PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (R394E) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, R394EPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F405G
Plasmid#169988PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F405G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, F405GPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM F370G
Plasmid#169986PurposeTet/Dox-inducible mammalian expression of CARD8 with a site III mutation (F370G) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, F370GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pInducer20 CARD8-HA PAM E274R
Plasmid#169984PurposeTet/Dox-inducible mammalian expression of CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseLentiviralTagsHAExpressionMammalianMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND F405G + S297A
Plasmid#169974PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, F405GPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND E274R + S297A
Plasmid#169968PurposepDONR221 for Gateway shuttling. Contains the CARD8 autoprocessing-deficient FIIND (L162M-P434, no stop codon) that cannot bind DPP9 at site I (E274R)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND R394E + S297A
Plasmid#169972PurposepDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationS297A, R394EPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 PAM E274R
Plasmid#169977PurposepDONR221 for Gateway shuttling. Contains CARD8 that cannot bind DPP9 due to a mutation at site I (E274R) with sgRNA binding site mutationsDepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPAM, E274RPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG R394E + S297A
Plasmid#169955PurposeMammalian expression of CARD8-FLAG with a site III mutation (R394E) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, R394EPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG F405G + S297A
Plasmid#169957PurposeMammalian expression of CARD8-FLAG with a site III mutation (F405G) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationS297A, F405GPromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG E274R + S297A
Plasmid#169951PurposeMammalian expression of CARD8-FLAG that cannot bind DPP9 due to a mutation at site I (E274R) with an additional autoprocessing mutation (S297A)DepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationE274R, S297APromoterCMVAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only