Addgene

We narrowed to 13,070 results for: ung

Showing: 2541 - 2560 of 13070 results

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pJBL7086

Plasmid

#167250

Purpose

orthogonal Mango reporter output for cell-free expression

Depositor

Julius Lucks

Article

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-Mango

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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gCBEv3

Plasmid

#220618

Purpose

vector for encoding an engineered glycosylase-based cytosine (C) base editor (gCBEv3) driven by EF1a promoter, guide RNAs compatible with SpG driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

et al Nat Commun 15

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;N213D,K184A,A214V), SpCas9(D10A)

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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gTBEv4

Plasmid

#220620

Purpose

vector for encoding an engineered glycosylase-based thymine (T) base editor (gTBEv4) driven by EF1a promoter, guide RNAs compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

Depositor

Huawei Tong

Article

et al Nat Commun 15

Insert

nSpCas9(Nterm)-XTEN-hUNG2-XTEN-Cas9(Cterm)

Use

Tags

Expression

Mammalian

Mutation

hUNG2(del1-88;Y156A,A214T,Q259A,Y284D), SpCas9(D1…

Promoter

hU6, EF1a

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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gWiz_BM40-vGAT-VL-hIgG1-CL

Plasmid

#218437

Purpose

mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

Depositor

Axel Brunger

Article

Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

Insert

vGAT-VL-hIgG1-CL

Use

Affinity Reagent/ Antibody

Tags

BM40

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pNS49 (enAsCas12a-KRAB piggyBac)

Plasmid

#217335

Purpose

piggyBac expresion of enAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

enAsCas12a

Use

CRISPR

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Expression

Mammalian

Mutation

E174R/S542R/K548R

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD101

Plasmid

#212925

Purpose

Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SUC2 (SUC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD043

Plasmid

#212913

Purpose

Encodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SCW4 (SCW4 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD084

Plasmid

#212921

Purpose

Encodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

TIP1 (TIP1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD140

Plasmid

#212927

Purpose

Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD080

Plasmid

#212917

Purpose

Encodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA1 (AGA1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD001

Plasmid

#212898

Purpose

Encodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

AGA2 (AGA2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD008

Plasmid

#212905

Purpose

Encodes S. cerevisiae PLB1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

PLB1 (PLB1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD006

Plasmid

#212903

Purpose

Encodes S. cerevisiae MFalpha1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

MFalpha1 (MF(ALPHA)1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD083

Plasmid

#212920

Purpose

Encodes the HA-tagged S. cerevisiae Sed1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

SED1 (SED1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYSD082

Plasmid

#212919

Purpose

Encodes the HA-tagged S. cerevisiae Cwp2p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

CWP2 (CWP2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD003

Plasmid

#212900

Purpose

Encodes S.cerevisiae ECM14 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

ECM14 (ECM14 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD002

Plasmid

#212899

Purpose

Encodes S.cerevisiae CRH1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

CRH1 (CRH1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD007

Plasmid

#212904

Purpose

Encodes S. cerevisiae OST1 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

OST1 (OST1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pYSD041

Plasmid

#212911

Purpose

Encodes the S. cerevisiae YAR066W Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

YAR066W (YAR066W Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD023

Plasmid

#212909

Purpose

Encodes a hybrid S. cerevisiae OST1 pre- MFalpha1 pro- signal peptide (OST1MFapp) as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

MFalpha1 (MF(ALPHA)1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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