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Plasmid#192658PurposeA plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomatoDepositorInsertTdTomato
UseTagsExpressionMammalianMutationPromoterU6, TRE3GAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-miniCMV-Nluc
Plasmid#192657PurposeA plasmid encoding miniCMV sgRNA and miniCMV-promoter-NlucDepositorInsertNluc
UseLuciferaseTagsExpressionMammalianMutationPromoterU6, miniCMVAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-scFv-VP64_Blast
Plasmid#192655Purpose3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance markerDepositorInsertscFv-VP64
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf
Plasmid#182000PurposeCMV overexpression of SNAP-tag fast in mammalian cell linesDepositorInsertSTf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_NES_STf
Plasmid#182001PurposeCMV overexpression of SNAP-tag fast strictly in the cytoplasm of mammalian cell linesDepositorInsertNES-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_STf_SKL
Plasmid#182007PurposeCMV overexpression of SNAP-tag fast in the peroxisome of mammalian cell linesDepositorInsertSTf_SKL
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK26 pHomL-TEF2p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179010PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK25 pHomL-TDH3p-Neon(gfp)-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179009PurposeParent vector for markerless yeast integration cassettes with N-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-lacO-sfGFP
Plasmid#180756PurposepET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJP118
Plasmid#176494PurposeExpresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoterDepositorInsertLifeact peptide
UseCapsaspora owczarzaki expressionTagsExpressionMutationPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCJH026_proD_Cas12c-multiplexing_CAM
Plasmid#183073PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repressionDepositorInsertExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK85
Plasmid#176611PurposeTo minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.DepositorInsertSNAP-FCERG-mGFP
UseLentiviralTagsExpressionMammalianMutationC25APromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
NK83
Plasmid#176610PurposeTo measure Syk intensity in response to ligand clustering using DNA origamiDepositorInsertNeonGreen-tSH2 Syk (Syk Synthetic, Mouse)
UseLentiviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)
Plasmid#179080PurposeParent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminatorsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK42 pHomL-RPL18Bp-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179026PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK41 pHomL-TEF2p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179025PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK40 pHomL-TDH3p-GFPdropout-Link-3HA-SSA1t-HomR (AmpR)
Plasmid#179024PurposeParent vector for markerless yeast integration cassettes with C-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK39 pHomL-RPL18Bp-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179023PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK38 pHomL-TEF2p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179022PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK37 pHomL-TDH3p-3HA-Link-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179021PurposeParent vector for markerless yeast integration cassettes with N-terminal 3xHemagglutinin-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only