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pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-StefinA
Plasmid#182404PurposeBacterial expression of Vesicle Nucleating peptide6-stefinA fusionDepositorInsertVNp6-stefin (CSTA Human)
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
p4xSMAD_RE
Plasmid#118051PurposeGBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motifDepositorInsert4 copies the SMAD DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-Flag-SAMTOR
Plasmid#102420Purpose3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoterDepositorInsertSAMTOR (BMT2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterUBCAvailable sinceNov. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CABE-T1.17
Plasmid#193260PurposeExpression of CABE-T1.17 base editor in mammalian cells, CMV promoterDepositorInsertCABE-T1.17
UseTagsExpressionMammalianMutationD10A (Cas9), TadA mutations withinPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK2 WT
Plasmid#12182DepositorInsertMEKK2 (Map3k2 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-ARF
Plasmid#19930DepositorInsertcyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D
Plasmid#52521PurposeCo-expresses HA tagged channelrhodopsin and hM4D from a Cre-dependent virusDepositorInsertChR2-2a-hM4D
UseAAV and Cre/LoxTags2xHAExpressionMutationPromoterAvailable sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK4alpha WT
Plasmid#12187DepositorInsertMEKK4 (Map3k4 Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBeast-pAhpC-RBS- LacZ
Plasmid#190060PurposeLacZ containing colorimetric reporter plasmid expressing it under the control of the OxyR/H2O2 inducible pAhpC promoterDepositorInsertlacZ
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterAvailable sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rhoA QL
Plasmid#61632Purposemammalian expression of rhoA constitutively active mutantDepositorInsertrhoA (RHOA Human)
UseTagsExpressionMammalianMutationQ63L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTACR TH1-p2aGFP
Plasmid#135815PurposeCRISPR based on px330 p2aGFP targeting TH gene near stop codonDepositorInsertTH 1 guide
UseCRISPRTagsp2a Myr-eGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTACR TH2-p2aGFP
Plasmid#135816PurposeCRISPR based on px330 p2aGFP targeting TH gene near stop codonDepositorInsertTH 2 guide
UseCRISPRTagsp2a Myr-eGFPExpressionMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterial and PlantMutationPromoterUBQ10Available sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRTagsExpressionBacterial and PlantMutationPromoterAvailable sinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
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