Addgene

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pUPD2+AtAct2t

Plasmid

#170882

Purpose

Phytobrick - AtACT2t

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

AtACT2t

Use

Tags

Expression

Plant

Mutation

426C>T to remove BbsI restriction site

Promoter

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUG-131

Plasmid

#104713

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

MIEL1 (AT5G18650 Mustard Weed)

Use

Synthetic Biology

Tags

Expression

Mutation

BpiI/BsaI sites removed by point-mutation

Promoter

Available since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 R236A/L263A

Plasmid

#51589

Purpose

expresses RNAiR myc-tagged Rem2 non-Ca channel interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

R236A and L263A; silent mutations at shRNA 1 and …

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Rem2 S69A/S334A

Plasmid

#51588

Purpose

expresses RNAiR myc-tagged Rem2 non-14-3-3 interacting

Depositor

Suzanne Paradis

Article

Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

Insert

Rem2 (Rem2 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

S69A and S334A; silent mutations at shRNA 1 and 2…

Promoter

CMV

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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GMIP

Plasmid

#32046

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

GMIP (GMIP Human)

Use

Tags

His (with TEV cleavage)

Expression

Bacterial

Mutation

amino acids 80-357 only

Promoter

Available since

Jan. 13, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-FIP3

Plasmid

#74753

Purpose

Expressed sfGFP-tagged FIP3 in E. coli for protein production

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

Rab11 family interacting protein 3 (RAB11FIP3 Human)

Use

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

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pZG61

Plasmid

#202575

Purpose

RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Use

Tags

Expression

Plant

Mutation

E488Q

Promoter

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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StrepII-tag

Plasmid

#55181

Purpose

Streptactin-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

StrepII tag

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag mPTIP

Plasmid

#15557

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
GFP-Tollip

Plasmid

#178050

Purpose

GFP fused to human Tollip cDNA. Localisation and expression in mammalian cells.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE2-Bla(HA–RILP)

Plasmid

#102425

Purpose

HA tag fused to the N-terminus of RILP for the expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Tet-responsive

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH-p18-CIBN

Plasmid

#188990

Purpose

A lentiviral plasmid encoding lysosome-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

p18

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAct-PE2-HygroR

Plasmid

#149552

Purpose

Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

Tags

Expression

Insect

Mutation

Promoter

Act5c

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pWZLneo Flag mPTIP

Plasmid

#15558

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

PTIP (Paxip1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

bactin2

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-stop

Plasmid

#117243

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Article

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Insert

CIBN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSumo-His6- Sumo-AtTPL(1-209)

Plasmid

#177856

Purpose

Bacterial Expression of TPL

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPL (TPL Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-WIPI-1

Plasmid

#38273

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only