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pMinDis.X513-iNicSnFR3a-(CC93)-ER

Plasmid

#125121

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(CC93)-ER

Use

Tags

ER retention motif

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pMinDis.X513-iNicSnFR3b-(V7)-ER

Plasmid

#125123

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(V7)-ER

Use

Tags

Fusion protein -Endoplasmic retention motif

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHM.X513-iNicSnFR3a-(CC93)

Plasmid

#124880

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR3a-(CC93)

Use

Tags

HA Tag and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHHM.X513-iNicSnFR2-(CC90)

Plasmid

#124836

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotine

Depositor

Henry Lester

Article

Shivange et al J Gen Physiol. 2019 Feb 4. pii: jgp.2018

Insert

iNicSnFR2-(CC90)

Use

Tags

HA tags and His Tag

Expression

Bacterial

Mutation

NA

Promoter

T7

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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UASp-deltaN cycB

Plasmid

#112967

Purpose

P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Lee et al Genetics. 2001 Aug;158(4):1545-56.

Insert

cycB (CycB Fly)

Use

P insertion vector

Tags

Expression

Mutation

N-term deletion of first 46 amino acids, includes…

Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL6

Plasmid

#67464

Purpose

Mammalian expression of hArl6 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6

Plasmid

#67328

Purpose

hArl6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CS2+MYC-Etv5:T139D

Plasmid

#49097

Purpose

expression of Etv5 T139D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 T139D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

MYC

Expression

Mammalian

Mutation

T139D

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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Cs2+Etv5 S142D

Plasmid

#49096

Purpose

expression of Etv5 S142D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv5 S142 D (etv5a Zebrafish)

Use

Xenopus/avian/zebrafish

Tags

Expression

Mammalian

Mutation

S142D

Promoter

CMV

Available since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
psicheck-2-Casp8-mod

Plasmid

#48171

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> ATT) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of CASP8 (CASP8 Human)

Use

Luciferase

Tags

Expression

Mutation

G/T MUTATION IN ATG OF UORF

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Mc2r-mod

Plasmid

#48173

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of MC2R (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

A/G MUTATION IN ATG OF UORF

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Kdr-mod

Plasmid

#48177

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> GTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Tags

Expression

Mutation

A/G MUTATION IN ATG OF UORF

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2-mod

Plasmid

#48180

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

A/T MUTATION IN ATG OF UORF

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
psicheck-2-Hsdl2

Plasmid

#48179

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of HSDL2 (Hsdl2 Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pBabe_puro_DEST_Flag_LRRC31

Plasmid

#45271

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

LRRC31 (LRRC31 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
alpha 0-CFP in pdest12.2

Plasmid

#36312

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein alpha 0 (Gnao1 Rat)

Use

Tags

CFP

Expression

Mammalian

Mutation

Introduced KpnI and SpeI sites between 92nd and 9…

Promoter

CMV

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a I18A

Plasmid

#40066

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Isoleucine 18 to Alanine

Promoter

Tac

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T-1 hSlp4-a W118S

Plasmid

#40065

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a W118S (SYTL4 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Tryptophane 118 to Serine

Promoter

Tac

Available since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-W3083F

Plasmid

#18076

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-2TK-WW-CR-W3083F/P3086A

Plasmid

#18077

Depositor

Marius Sudol

Article

Rentschler et al Biol Chem. 1999 Apr . 380(4):431-42.

Insert

Dystrophin (DMD Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Second conserved Tryptophan (W) of the WW domain …

Promoter

Available since

May 29, 2008

Availability

Academic Institutions and Nonprofits only