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Addgene
Showing: 2541 - 2560 of 16002 results

  1. pQCXIP-SHMY-PAUF

    Plasmid
    #182645
    Purpose
    Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    Pancreatic adenocarcinoma upregulated factor (PAUF)
    Use
    Retroviral
    Tags
    His6-tag, N-terminal ER targeting sequence of hem…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182645-plasmid-map-sequence-id-358618

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  2. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  3. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Use
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  6. pJYDN3

    Plasmid
    #162456
    Purpose
    Yeast display platform plasmid N3 which allows for simultaneous expression of the protein of interest at the N-terminus of Aga2p yeast agglutinin and eUnaG2 or nbALFA detection.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    ALFA tag, HA, myc tags, and eUnaG2 fluorescent pr…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162456-plasmid-map-sequence-id-321464

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  7. pJYDC1

    Plasmid
    #162458
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter, eUnaG2 bilirubin dependent green-yellow fluorescent protein, is fused to the Nterminus
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA tag, HDEL sequence, eUnaG2 fluorescent protein…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162458-plasmid-map-sequence-id-321474

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  8. pJYDC3

    Plasmid
    #162460
    Purpose
    Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    162460-plasmid-map-sequence-id-330057

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  10. BFP-RanQ69L

    Plasmid
    #163438
    Purpose
    Mammalian expression of RanQ69L with tag-BFP; contains miniAux domain for degredation
    Depositor
    Sanford Simon
    Insert
    RanQ69L (RAN Human)
    Use
    Tags
    tag-BFP
    Expression
    Mammalian
    Mutation
    Q69L
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    163438-plasmid-map-sequence-id-321655

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  11. pET-Bst-LF-Idaho

    Plasmid
    #170469
    Purpose
    Expresses His-tagged Bst-LF-Idaho in E. coli
    Depositor
    Jonathan Schmid-Burgk
    Insert
    Bst-LF-Idaho
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    170469-plasmid-map-sequence-id-338354

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  12. His-GST

    Plasmid
    #215169
    Purpose
    Encodes the synthetic glutathionine S-transferase with N-terminal His tag
    Depositor
    Travis Beddoe, Bhuvana Shanbhag
    Insert
    His-GST
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Availability
    Academic Institutions and Nonprofits only
    215169-plasmid-map-sequence-id-430165

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  13. pET24a MDH_STRAW

    Plasmid
    #204240
    Purpose
    Bacterial expression of S. avermitilis malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_STRAW (mdh Streptomyces avermitilis)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    Lac
    Availability
    Academic Institutions and Nonprofits only
    204240-plasmid-map-sequence-id-413348

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  16. pCHA_OROV_NSm

    Plasmid
    #208940
    Purpose
    Expresses C-terminally HA-tagged Oropouche orthobunyavirus NSm protein from CMV promoter in mammalian cells
    Depositor
    Gerald Barry
    Insert
    NSm
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 949 bp from 5' end (Gn), deleted 29…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    208940-plasmid-map-sequence-id-419028

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  17. pGBW-m4252821

    Plasmid
    #153564
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    153564-plasmid-map-sequence-id-313659

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  18. pGBW-m4252959

    Plasmid
    #153578
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    153578-plasmid-map-sequence-id-300166

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  19. pGBW-m4252822

    Plasmid
    #153716
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    153716-plasmid-map-sequence-id-297405

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  20. pGBW-m4252944

    Plasmid
    #153811
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 E (envelope)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
    153811-plasmid-map-sequence-id-307379

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Showing: 2541 - 2560 of 16002 results