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Plasmid#200895PurposeExpresses human codon-optimized V. cholerae Cas6 from a CMV promoter.DepositorInsertVchCas6
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL2622 (pCas7_Vch)
Plasmid#200894PurposeExpresses human codon-optimized V. cholerae Cas7 from a CMV promoter.DepositorInsertVchCas7
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorInsert53BP1(1208-1977) (TP53BP1 Human)
UseTags6x-His; mCherryExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-GFP-mCherry-3pGW
Plasmid#53965PurposeBidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherryDepositorInsertsGFP
mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF20
Plasmid#52645Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF20
UseTagsExpressionMammalianMutationD362N/D366N mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF16
Plasmid#52642Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF16
UseTagsExpressionMammalianMutationD431N/D433N/D435N/E442A mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF19
Plasmid#52644Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF19
UseTagsExpressionMammalianMutationT328D/T330D mutated from GCaMP3PromoterT7Available sinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-GFPd2
Plasmid#115665PurposePiggybac transposon plasmid for destabilized eGFP (GFPd2)DepositorInsertGFPd2
UseSynthetic BiologyTagsDestabilizationExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-VSFP-CR
Plasmid#40257DepositorInsertVSFP-CR
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMito-dCherry-FRB
Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
UseTagsExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAM19A2-V5
Plasmid#184338PurposeExpression of V5-tagged mouse FAM19A2DepositorInsertFAM19A2 (Tafa2 Mouse)
UseTagsV5-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL3
Plasmid#114673PurposeT7 vector having very low basal expression of target protein can clone and express almost any gene. Asymmetric ligation clones as many as 3 genes simultaneously for co-expression from the same mRNA.DepositorTypeEmpty backboneUseTagsoptional Ser-Gly-6HisExpressionBacterialMutationPromoterT7Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-EF05
Plasmid#52558Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-EF05
UseTagsExpressionInsectMutationchanged from GCaMP3: D397N/G398A/N399DPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBRC169
Plasmid#79670PurposeDsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (169–225)/RC169ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGN155
Plasmid#74992PurposeGFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsDepositorTypeEmpty backboneUseReporter plasmidTagsGFP (1–155)/GN155ExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
AEB-TMR10pUC19
Plasmid#63858PurposeThis pUC19 vector contains a tetramethylrosamine (TMR) binding riboswitch, controlling the translation initaition rate of luciferase gene.DepositorInsertTMR10 TMR riboswitch
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS15
Plasmid#52652Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS15
UseTagsExpressionMammalianMutationdeleted G47/H48 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mHH
Plasmid#51057PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.DepositorInsertRGR-GFP
UseCRISPRTagsExpressionBacterial and YeastMutationPromoterYeast ADH1 promoterAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBCN155-MXMT
Plasmid#79815PurposeCFP-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsCN155/CFP (1–155)ExpressionPlantMutationPromoterCaMV 35S promoter and CaMV35SAvailable sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP-RS14
Plasmid#52651Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP-RS14
UseTagsExpressionMammalianMutationdeleted G47 from GCaMP3PromoterT7Available sinceMay 6, 2014AvailabilityAcademic Institutions and Nonprofits only