Addgene

We narrowed to 11,260 results for: pcDNA

Showing: 2521 - 2540 of 11260 results

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pCDNA3-V5-PTPN14-del-C

Plasmid

#61005

Purpose

Expresses human PTPN14 (del-phosphotase domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete C (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 934-1187

Promoter

CMV

Available since

March 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-siResist

Plasmid

#49852

Purpose

Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-ORF63-2xCSTREP

Plasmid

#136223

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF63

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF63

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+) Drp1 V2 S579D

Plasmid

#46343

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Use

Tags

Expression

Mammalian

Mutation

S579D

Promoter

CMV

Available since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pY116 (pcDNA3.1-huMb2Cpf1)

Plasmid

#92292

Purpose

Expression of humanized Mb2Cpf1

Depositor

Feng Zhang

Insert

huMb2Cpf1

Use

Tags

NLS and NLS-3xHA

Expression

Mammalian

Mutation

Promoter

Available since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HshnRNPR_AA

Plasmid

#148235

Purpose

Mammalian Expression of HshnRNPR

Depositor

Elisa Izaurralde

Insert

HshnRNPR (HNRNPR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon wt

Plasmid

#11942

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4beta

Plasmid

#15517

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

pCDNA3 Flag MKK4beta (MAP2K4 Human)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
pY018 (pcDNA3.1-hPdCpf1)

Plasmid

#69990

Purpose

Expresses humanized PdCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPdCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pri-let-7e

Plasmid

#51380

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

pri-let-7e (MIRLET7E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO HSPA6

Plasmid

#19459

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

HSPA6 (HSPA6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RIα-ICUE4

Plasmid

#181848

Purpose

ICUE4 cAMP sensor tethered to the PKA RIα subunit.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-ICUE4 (RAPGEF3, PRKAR1A Human)

Use

Tags

ECFP, PKA RIα, and cpVenus(L194)

Expression

Mammalian

Mutation

Glutamine 122 in Epac1 domain mutated to glutamat…

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K8a-2xCSTREP

Plasmid

#136158

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8a

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8a

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CFP-KRAS4B

Plasmid

#112717

Purpose

Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

CFP-KRAS4B (KRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-optoB2-SS-YFP

Plasmid

#75270

Purpose

Arrestin independent version of optoB2 with serine mutations at S362A,S363G

Depositor

Michael Bruchas

Article

Siuda et al Nat Commun. 2015 Sep 28;6:8480. doi: 10.

Insert

Arrestin independent version of optoB2

Use

Tags

eGFP

Expression

Mammalian

Mutation

serine mutations at S362A,S363G

Promoter

Available since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Use

Tags

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_PA-Cre-Y324F

Plasmid

#122961

Purpose

Catalytically inactive mutant of PA-Cre

Depositor

Moritoshi Sato

Article

Kawano et al Nat Chem Biol. 2016 Dec;12(12):1059-1064

Insert

pcDNA3.1_PA-Cre-Y324F

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-neo-Strep_Flag_Cterm

Plasmid

#102645

Purpose

Epitope tag c-terminus with SBP-Flag moiety

Depositor

Michael E. Wright

Article

Hsiao et al BMC Cancer. 2015 Mar 31;15:204. doi: 10.

Type

Empty backbone

Use

Tags

SBP-Flag, MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREPG…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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