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Showing: 2521 - 2540 of 3045 results

  2. Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A, E1707A) pCAGGS

    Plasmid
    #206115
    Purpose
    expression of rat Cav2.1 with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (Cacna1a Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E320A, E670A, E1411A, E1707A
    Promoter
    CMV/B-actin
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    206115-plasmid-map-sequence-id-415931

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  4. pGBT-RP8.1

    Plasmid
    #189846
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    mRFP and tagBFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189846-plasmid-map-sequence-id-373953

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  5. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid
    #206098
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV/B-actin
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206098-plasmid-map-sequence-id-415670

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  6. pGBT-RP2.2

    Plasmid
    #189842
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189842-plasmid-map-sequence-id-373952

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  7. pGBT-RP2.3

    Plasmid
    #189843
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    GFP GM2 variant and mRFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189843-plasmid-map-sequence-id-373951

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  8. pGBT-RP8.3

    Plasmid
    #189848
    Purpose
    Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.
    Depositor
    Stephen Ekker
    Article
    Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1
    Type
    Empty backbone
    Use
    Cre/Lox
    Tags
    mRFP and tagBFP
    Expression
    Mutation
    Promoter
    beta Actin
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    189848-plasmid-map-sequence-id-373955

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  13. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Tags
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  14. pCag SE (Self-excising) FlpO-2A-Cre EV

    Plasmid
    #130986
    Purpose
    episomal expression of FlpO and Cre recombinases, self excising
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    FlpO-2A-Cre
    Use
    Cre/Lox and Unspecified; Episomal expression vect…
    Tags
    Expression
    Mammalian
    Mutation
    protamine intron added to FlpO to prevent bacteri…
    Promoter
    CMV/Chick β-actin (CAG)
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130986-plasmid-map-sequence-id-304997

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  15. NK690

    Plasmid
    #120849
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    Jumble-mWasabi
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120849-plasmid-map-sequence-id-233698

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  16. NK676

    Plasmid
    #120852
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    pac-P2A-mTFP
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    120852-plasmid-map-sequence-id-233741

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  17. NK696

    Plasmid
    #120855
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    pac-P2A-mTFP-Jumble
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    120855-plasmid-map-sequence-id-233745

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  18. NK694

    Plasmid
    #120853
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    pac-P2A-Jumble-mTFP
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    120853-plasmid-map-sequence-id-233742

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  19. NK691

    Plasmid
    #120850
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    Jumblelw1-mWasabi
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Jumblelw1 allele: T to C at nt 1109 causes L305P …
    Promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    120850-plasmid-map-sequence-id-233701

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  20. NK697

    Plasmid
    #120856
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Insert
    pac-P2A-mTFP-Jumblelw1
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Jumblelw1 allele: T to C at nt 1109 causes L305P …
    Promoter
    Available since
    Jan. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    120856-plasmid-map-sequence-id-233746

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Showing: 2521 - 2540 of 3045 results