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LCV2 PQBP1 KO1

Plasmid

#217447

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human PQBP1

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

sgRNA targeting PQBP1 (PQBP1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse cGAS #1-gRNA-Cas9-Puro

Plasmid

#186890

Purpose

gRNA targeting mouse cGAS (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Cgas Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-mouse cGAS #2-gRNA-Cas9-Puro

Plasmid

#186891

Purpose

gRNA targeting mouse cGAS (with Cas9 insert)

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Cas9 (Cgas Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-mouse cGAS-HA

Plasmid

#186875

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS with C-terminal HA tag (Cgas Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-mouse cGAS-GFP

Plasmid

#186877

Purpose

Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Mouse cGAS with C-terminal GFP fusion (Cgas Mouse)

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-gibbon cGAS-HA

Plasmid

#186862

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Gibbon cGAS with C-terminal HA tag (CGAS Hylobatidae)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

TRE3G

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pKM-U6-reRNA-hsEMX1

Plasmid

#205637

Purpose

reRNA guide targeting Homo sapiens EMX1

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-hsEMX1 (EMX1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm2

Plasmid

#222919

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 2 (DAZL Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_DAZL_cterm3

Plasmid

#222920

Purpose

Cas9/sgRNA plasmid for targeting DAZL

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, DAZL sgRNA 3 (DAZL Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_TFAP2C_cterm

Plasmid

#222916

Purpose

Cas9/sgRNA plasmid for targeting TFAP2C

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, TFAP2C sgRNA (TFAP2C Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pX330_FIGLA_cterm4

Plasmid

#222909

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 4 (FIGLA Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_FIGLA_cterm3

Plasmid

#222908

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 3 (FIGLA Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_FIGLA_cterm2

Plasmid

#222907

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 2 (FIGLA Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_FIGLA_cterm1

Plasmid

#222906

Purpose

Cas9/sgRNA plasmid for targeting FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, FIGLA sgRNA 1 (FIGLA Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pX330_NANOS3_cterm

Plasmid

#222904

Purpose

Cas9/sgRNA plasmid for targeting NANOS3

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

Cas9, NANOS3 sgRNA (NANOS3 Human, Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-FIGLA

Plasmid

#222929

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of FIGLA

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

FIGLA (FIGLA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DLX5

Plasmid

#222927

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DLX5

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

DLX5 (DLX5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERH-LHX8

Plasmid

#222921

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of LHX8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

LHX8 (LHX8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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AA320

Plasmid

#215952

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

rev{U6_v1; DR_v0 [EnAs]; sgCD47_v1 [EnAs]; DR_v1 [EnAs]; sgCD47_v2 [EnAs]}

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge