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Plasmid#207122PurposeLentiviral plasmid to express mouse Mettl3DepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationPromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10A(E203Q)-HA
Plasmid#209258PurposeMammalian expression of ATP10A mutant E203QDepositorInsertATP10A (ATP10A Human)
UseRetroviralTagsHAExpressionMutationE203QPromoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10A(E203Q)-HA
Plasmid#209246PurposeMammalian expression of ATP10A mutant E203QDepositorInsertATP10A (ATP10A Human)
UseTagsHAExpressionMammalianMutationE203QPromoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3a-UBE2G2
Plasmid#204209PurposeBacterial expression of UBE2G2DepositorInsertUBE2G2 (Ube2g2 Mouse)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_RV-B14_VP1
Plasmid#202074PurposeBacterial expression of VP1 protein from rhinovirus B14 (RV-B14). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-B14 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_RV-A89_VP1
Plasmid#202073PurposeBacterial expression of VP1 protein from rhinovirus A89 (RV-A89). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertRV-A89 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.11_Nued2_E5
Plasmid#203834PurposeLuciferase reporter plasmid for Igf1 enhancer 5DepositorInsertE5 (Igf1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T_EV-D68_VP1
Plasmid#202072PurposeBacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.DepositorInsertEV-D68 VP1 protein with Histag
UseTags6xHis-tag and GSTExpressionBacterialMutationPromotertacAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2trLATo
Plasmid#203746PurposeEncodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.DepositorInserttrLATo (Lat Mouse)
UseTagsER translocation signal - mEos3.2ExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-sCAG-GluClα.CreON
Plasmid#196995PurposeCre recombinase dependent expression of GluClv2.0 alpha subunit. GluClα contains a Cerulean tag. When co-expressed with GluClv2.0 beta subunit, agonist (Ivermectin) induces neuronal silencing.DepositorInsertGluClα -Cerulean
UseAAV and Cre/LoxTagsCeruleanExpressionMutationPromotershort CAGAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPIP1;4-NLS-3xmCitrine
Plasmid#192524PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertPLASMA MEMBRANE INTRINSIC PROTEIN 1;4 (PIP1;4 Mustard Weed)
UseTags3xmCitrine and NLSExpressionPlantMutationPromoterPIP1;4Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pegRNA_HEK4_1bp-insertion
Plasmid#201977PurposeExpress a pegRNA used for 1bp insertion at HEK4 siteDepositorInsertpegRNA_HEK4_1bp-insertion
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-R249H_X
Plasmid#147979PurposeInsect Expression of DmCaf40-R249HDepositorInsertDmCaf40-R249H (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-Y208AR210AH213AA253F_X
Plasmid#147980PurposeInsect Expression of DmCaf40-Y208AR210AH213AA253FDepositorInsertDmCaf40-Y208AR210AH213AA253F (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-Y208AR249A_X
Plasmid#147981PurposeInsect Expression of DmCaf40-Y208AR249ADepositorInsertDmCaf40-Y208AR249A (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-A253FY208A_X
Plasmid#147992PurposeInsect Expression of DmCaf40-A253FY208ADepositorInsertDmCaf40-A253FY208A (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-R249H_X
Plasmid#147994PurposeInsect Expression of DmCaf40-R249HDepositorInsertDmCaf40-R249H (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-Y208AR249A_X
Plasmid#147995PurposeInsect Expression of DmCaf40-Y208AR249ADepositorInsertDmCaf40-Y208AR249A (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-F65AC69Y_X
Plasmid#147974PurposeInsect Expression of DmCaf40-F65AC69YDepositorInsertDmCaf40-F65AC69Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-N63AF65AC69Y_X
Plasmid#147975PurposeInsect Expression of DmCaf40-N63AF65AC69YDepositorInsertDmCaf40-N63AF65AC69Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76Y_X
Plasmid#147976PurposeInsect Expression of DmCaf40-N63AF65AC69YV76YDepositorInsertDmCaf40-N63AF65AC69YV76Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-N63AF65AC69YV76YR210AH213A_X
Plasmid#147977PurposeInsect Expression of DmCaf40-N63AF65AC69YV76YR210AH213ADepositorInsertDmCaf40-N63AF65AC69YV76YR210AH213A (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-NFCVYRHAtoAAYYAAAF_X
Plasmid#147978PurposeInsect Expression of DmCaf40-NFCVYRHAtoAAYAAAFDepositorInsertDmCaf40-NFCVYRHAtoAAYAAAF (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-NFCVYAtoAAYYAF_W
Plasmid#147829PurposeInsect Expression of DmCaf40-NFCVYAtoAAYYAFDepositorInsertDmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT-CTAP-PrP-SBP-DmCaf40_W
Plasmid#147832PurposeInsect Expression of DmCaf40DepositorInsertDmCaf40 (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-NFCVYAtoAAYYAF_W
Plasmid#147838PurposeInsect Expression of DmCaf40-NFCVYAtoAAYYAFDepositorInsertDmCaf40-NFCVYAtoAAYYAF (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-R210AH213A_W
Plasmid#147839PurposeInsect Expression of DmCaf40-R210AH213ADepositorInsertDmCaf40-R210AH213A (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-luc-TNFalpha-ARE-polyA-Malat_AH
Plasmid#148878PurposeMammalian Expression of TNFalpha-ARE-polyA-MalatDepositorInsertTNFalpha-ARE-polyA-Malat (TNF Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmMe31B-R307AK313AK314A-dsRNAres_AC
Plasmid#148392PurposeInsect Expression of DmMe31B-R307AK313AK314ADepositorInsertDmMe31B-R307AK313AK314A (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-QHTKRKKtoAAAAAAA_AC
Plasmid#148396PurposeInsect Expression of DmMe31B-QHTKRKKtoAAAAAAADepositorInsertDmMe31B-QHTKRKKtoAAAAAAA (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-R307AK313AK314A_AC
Plasmid#148397PurposeInsect Expression of DmMe31B-R307AK313AK314ADepositorInsertDmMe31B-R307AK313AK314A (me31B Fly)
UseTagsExpressionInsectMutationone silent mutation A1101G compared to the sequen…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1147-1673_AC
Plasmid#148398PurposeInsect Expression of DmNot1_1147-1673DepositorInsertDmNot1_1147-1673 (Not1 Fly)
UseTagsExpressionInsectMutation2 silent mutations compared to the sequence given…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1377-1673_AC
Plasmid#148400PurposeInsect Expression of DmNot1_1377-1673DepositorInsertDmNot1_1377-1673 (Not1 Fly)
UseTagsExpressionInsectMutation1 silent mutation compared to the sequence given …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot1iso1_1085-1605_AC
Plasmid#148413PurposeMammalian Expression of HsNot1iso1_1085-1605DepositorInsertHsNot1iso1_1085-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_AC
Plasmid#148417PurposeMammalian Expression of HsRckDepositorInsertHsRck (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_1-295_AC
Plasmid#148418PurposeMammalian Expression of HsRck_1-295DepositorInsertHsRck_1-295 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsRCK-E236Q_H
Plasmid#146456PurposeMammalian Expression of HsRck-E236QDepositorInsertHsRck-E236Q (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-mNeon
Plasmid#186605PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to mNeonGreenDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsRck_296-472_AC
Plasmid#148419PurposeMammalian Expression of HsRck_296-472DepositorInsertHsRck_296-472 (DDX6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hASCL1 gRNA_1-MS2-Puro
Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL1RN gRNA_1-MS2-Puro
Plasmid#192677PurposeLentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNA #1 (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Immune checkpoint (library 2) Backbone
Plasmid#192363PurposePlasmid vector for in vivo immune checkpoint screening library (for checkpoint library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
T cell activation (library 1) Backbone
Plasmid#192364PurposePlasmid vector for in vitro Jurkat activation screening (for immune library)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 2
Plasmid#192365PurposetRNA sequence provider (for checkpoint library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 2
Plasmid#192366PurposetRNA sequence provider (for checkpoint library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Gly-tRNA-gRNA scaffold for library 1
Plasmid#192368PurposetRNA sequence provider (for immune library)DepositorInsertGly tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pro-tRNA-gRNA scaffold for library 1
Plasmid#192369PurposetRNA sequence provider (for immune library)DepositorInsertPro tRNA-gRNA scaffold
UseFor golden gate assemblyTagsExpressionMutationPromoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRFP-PA-GFP-RhoAQ63L
Plasmid#187282PurposeExpress pmRFP-PAGFP-RhoA Q63LDepositorInsertRhoA (RHOA Human)
UseTagsmRFP-PAGFPExpressionMammalianMutationRhoA Q63LPromoterCMV, T7Available sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only