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Plasmid#141150PurposeFluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cellsDepositorInsertOMP25 (Synj2bp Rat)
UseTagsAcGFPExpressionMammalianMutationOMP25 c terminus from addgene plasmid #69598PromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorArticleInsertHalotag-CNA35
UseTagsHalotagExpressionBacterialMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-dTAG-Blast
Plasmid#207716PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TFORF3431
Plasmid#144907PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C2 (NR3C2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0090
Plasmid#142456PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZEB1 (ZEB1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSU80
Plasmid#173834PurposeExpresses 80 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bRCC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Aurora A
Plasmid#198402PurposeExpression of EGFP-tagged Aurora A in mammalian cellsDepositorInsertAurora A
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceMay 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
anti-FX(a) Emi HC
Plasmid#113665PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FX/FXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/hChR2(H134R)-mCherry
Plasmid#20938PurposeMammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activationDepositorInsertchannelrhodopsin-2
UseTagsExpressionMammalianMutationH134R mutation in ChR2PromoterCMVAvailable sinceJune 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-mEGFP-HMGB1-WT
Plasmid#194543PurposeBacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion ProteinDepositorInsertHMGB1 (HMGB1 Human)
UseTagsmEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSU100
Plasmid#173835PurposeExpresses 100 kD Penn State ladder proteinDepositorInsertSTRHSTPABPACIL1bQRS
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
InsRA-TagBFP
Plasmid#79791PurposeInsulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagsTagBFP extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn-CaM-V5-sTb(N)
Plasmid#219783PurposesTb(N) half of CaST for neuron expressionDepositorInsertCaM-V5-sTb(N)
UseTagsV5ExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
PA-Dre
Plasmid#169706PurposePhotoactivatable Dre recombinaseDepositorInsertDre246-nMag-T2A-pMag-Dre247
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-QF2
Plasmid#83307Purposecodes for QF2 (truncated QF) in a gateway middle entry backbone.DepositorInsertQF
UseTagsExpressionMutationPromoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
Emi LC
Plasmid#113666PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with anti-FIX(a) and anti-FX(a) Emi HC's.DepositorInsertEmicizumab light chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-OKS-iG
Plasmid#58974PurposeRNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORFDepositorUseVenezuelan equine encephalitis (vee) virus rna re…TagsExpressionMammalianMutationPromoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Crimson
Plasmid#70683PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. Lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Crimson Reporter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEf1-a and hU6Available sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-M1
Plasmid#23007DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Rab11a-7
Plasmid#55124PurposeLocalization: Ras GTPase, Excitation: 587, Emission: 610DepositorInsertRab11a (RAB11A Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
Plasmid#221235PurposeTet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.DepositorInsertTadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPSU60
Plasmid#173833PurposeExpresses 60 kD Penn State ladder proteinDepositorInsertHSTPABPACRCC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT305
Plasmid#128801PurposeHuman codon optimized dCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backboneDepositorInsertdCas9-Dnmt3a-Dnmt3l-T2A-eGFP + gRNA expression cassette + LV backbone
UseCRISPR and LentiviralTagsExpressionMutationPromoterhUBC promoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_201
Plasmid#91105PurposeTransformation, Type: T-DNA, Plant Selection: none, Viral Replication: BeYDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterNo PromoterAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD276-Fc(DAPA)-AviTag-6xHis
Plasmid#156861PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertCD276 (CD276 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL CAG NFlag BAF170 ires GFP
Plasmid#24562DepositorInsertBAF170 (Smarcc2 Mouse)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceAug. 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGD425
Plasmid#132381Purposecontains the sequence of mTurquoise2 for C-term fusions in the pGGD000 backbone (GreenGate)DepositorInsertlinker-mTurquoise2
UseGreengate entry plasmidTagsExpressionMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorInsertTEK receptor tyrosine kinase (TEK Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
APEX2-GBP
Plasmid#67651PurposeMammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).DepositorInsertAPEX2-GBP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
HIP-flash (HOP-flash mutant)
Plasmid#83466PurposeNegative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRXTN
Plasmid#47916PurposepRXTN is a modified version of pRX-tight Puro (part of the two plasmid Tet-ON system from Clontech) encoding NAT instead of PAC.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterTetAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABEhygroHigheGFPhTERT
Plasmid#28169DepositorInserteGFPhTERT
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3
Plasmid#209727PurposeExpresses cytosolic medium affinity ratiometric ATP sensorDepositorInsert(cyto).iATPSnFR2.A95K.miRFP670nano3
UseLentiviralTagsExpressionMammalianMutationPromoterCamKIIAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSV Sport SREBP-1c dom neg
Plasmid#8885DepositorInsertSREBP-1c (Srebf1 Rat)
UseTagsExpressionMammalianMutationY305A amino acid substitution in the active versi…PromoterAvailable sinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
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mycBioID2-pBABE-puro
Plasmid#80900PurposeTo fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagshumanized A. aeolicus BioID2 with R40G and mycExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
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AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR
Plasmid#60226PurposeExpresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Renilla luciferase
Cre recombinase
UseAAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…TagsCre-HA and Rluc-P2AExpressionMammalianMutationPromoterEFS and U6Available sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-3c-rhVTN_FL
Plasmid#30223DepositorInsertrhVitronectin without deletions (VTN Human)
UseTagsExpressionBacterialMutationNo deletionsPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJEC819
Plasmid#190815PurposeCRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.DepositorInsertdCas9-XTEN-rpoA
UseCRISPR and Synthetic BiologyTagsXTEN (linker), followed by the N-terminal domain …ExpressionMutationPromoterSP30Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC28A3
Plasmid#132151PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC28A3 (SLC28A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorInsertNACH7 (CHRNA7 Human)
UseSynthesizedTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
anti-FIX(a) Emi HC
Plasmid#113664PurposeFor transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.DepositorInsertEmicizumab anti-FIX/FIXa heavy chain
UseTagsExpressionMammalianMutationPromoterCMV PromoterAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK3_038
Plasmid#123752PurposeEncodes R-Flinc-A, a cAMP reporter, as a Type 3 part to be used in the MTK systemDepositorInsertR-FlincA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 - hSRSF6
Plasmid#120419Purposeoverexpression of human SRSF6DepositorInsertSRSF6 (SRSF6 Human)
UseTagsExpressionMammalianMutationPromoterCMV immediate/earlyAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPSU150
Plasmid#173836PurposeExpresses 150 kD Penn State ladder proteinDepositorInsertSTRHSTPABMBPpepN
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T1-14-3-3 gamma GST
Plasmid#13280DepositorInsert14-3-3 gamma (Ywhag Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only