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Plasmid#100558PurposeExpresses EMX1* sgRNA. Target sequence: (G)GAGTCCGAGCAGAAGAAGAA. Same target sequence as #100555, except with an additional 5' G.DepositorInsertEMX1 sgRNA
UseTagsExpressionMutationPromoterU6Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMarVF1
Plasmid#99579PurposeMinitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114DepositorInsertV. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
UseSuicide vector in v. fischeri es114TagsExpressionMutationPromoterAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+-Tornado-split-nLuc
Plasmid#212612PurposeFor the expression of a split nLuc that produces luminescence only when circularized. To test your IRES of interest, clone into the EcoRI, BsiWI sites.DepositorInsertTornado-CVB3-split-nLuc
UseLuciferaseTagsExpressionMammalianMutationC373T mutation in CVB3PromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV CEPIA2mt
Plasmid#58218PurposeGreen fluorescent indicator for calcium imaging in the mitochondriaDepositorInsertCEPIA2mt
UseTagsmycExpressionMammalianMutationGCaMP2 N263Y E282V M339LPromoterCMVAvailable sinceJuly 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-HA-hYTHDC1
Plasmid#85167PurposeExpression of FLAG-HA-tagged human YTHDC1DepositorInsertYTHDC1 (YTHDC1 Human)
UseTagsFlag and HaExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRTP801/TA-ODD
Plasmid#206212PurposeAn expression vector of hypoxia-responsive transactivator under control of RTP801 promoterDepositorInserttetracycline-responsive transactivator fused with oxygen-dependent degradation domain
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSL4393 (pClpX_Eco)
Plasmid#200907PurposeExpresses human codon-optimized E. coli ClpX from a CMV promoter.DepositorInsertEcoClpX
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL3713 (pTnsC_Pse)
Plasmid#200900PurposeExpresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.DepositorInsertPseTnsC
UseTags3xNLSExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pY2
Plasmid#218115PurposeActivity reporter backbone with an empty protease cassette (BsaI) under (lexA-box)3PminCYC1 promoter and an empty substrate cassette (BsmBI) under pGAL1 promoter.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCIS GEM-CEPIA1er
Plasmid#58217PurposeRatiometric (Blue to Green) fluorescent indicator for calcium signaling in the endoplasmic reticulumDepositorInsertGEM-CEPIA1er
UseTagsmycExpressionMammalianMutationGEM-GECO1 E298D F359W D400E E407DPromoterCAGAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28c(+)-YTHDF2
Plasmid#102275PurposeFor bacterial expression of His-tagged human YTHDF2DepositorInsertYTHDF2 (YTHDF2 Human)
UseTags6X HISExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL2620 (pTniQ_Vch)
Plasmid#200892PurposeExpresses human codon-optimized V. cholerae TniQ from a CMV promoter.DepositorInsertVchTniQ
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSL0283 (pTnsABC)
Plasmid#130633PurposeExpresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.DepositorInsertVchTnsA, VchTnsB, VchTnsC
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
35S-DsREDmonomer-NosT
Plasmid#79183PurposeTransient expression vector for DsRED monomerDepositorTypeEmpty backboneUseReporter plasmidTagsDsREDmonomerExpressionPlantMutationPromoterCaMV35SAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Puro-CAG-ASAP1
Plasmid#52519PurposeFor mammalian expression of the fluorescent voltage sensor ASAP1DepositorInsertAccelerated Sensor of Action Potentials 1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28C-SARS-CoV-2-Nucleocapsid
Plasmid#204402PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsidDepositorInsertSARS-CoV-2 nucleocapsid (N SARS-CoV-2)
UseTags6x-HisExpressionBacterialMutationPromoterT7Available sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOD_009
Plasmid#159520PurposeRifampicin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with SalmonellaDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL2621 (pCas8_Vch)
Plasmid#200893PurposeExpresses human codon-optimized V. cholerae Cas8 from a CMV promoter.DepositorInsertVchCas8
UseTagsNLSExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only