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Plasmid#62321PurposesgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme design for yeast cellsDepositorInsertsgRNA +1x MS2, Pol II promoter with ribozyme cleavage
UseTagsExpressionYeastMutationPromoterpAdhAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBSSK p85 alpha (delta BH)
Plasmid#13431DepositorInsertp85 alpha (delta BH) (PIK3R1 Human)
UseTagsFLAGExpressionBacterialMutationBH domain replaced by flag tag.PromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
Lentiviral CaMKKbeta K193A
Plasmid#33323DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)
UseLentiviralTagsFlagExpressionMutationK194APromoterhuman PGKAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV5-Fyn C3,6S
Plasmid#19979DepositorInsertFyn (FYN Human)
UseTagsExpressionMammalianMutationC3,6SPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(TPF)
Plasmid#15741DepositorInsertJnk1a1(APY) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationTyr-192PhePromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSG5-HA-CaMKKbeta rat FL WT
Plasmid#33321DepositorInsertCalcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)
UseTagsHAExpressionMammalianMutationS3V, G96R, and P108LPromoterSV40Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Flag-MCPIP1(1-453)
Plasmid#90106PurposeMammalian expression of Flag-MCPIP1(1-453)DepositorInsertMCPIP1 (ZC3H12A Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S(RBSmut)_Psyn-sgRNAmreB
Plasmid#149594Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
N-term Hec1-targeted Aurora B FRET sensor
Plasmid#45239Purposesensor targeted to N-terminus of Hec1DepositorInsertHec1, Aurora B FRET sensor
UseTagsAurora B FRET sensor and Hec1ExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Jag2ICD-HA pBacPAK8-3
Plasmid#17332DepositorInsertJagged2 (Jag2 Rat)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-EAF pBacPAK8
Plasmid#15323DepositorInsertELL Associated Factor (EAF1 Human)
UseBaculovirus constructionTags6x His and FLAGExpressionMutationPromoterpolyhedrinAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83,88,104A
Plasmid#58405PurposeExpresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysines 83, 88, and 104 to AlaninePromoterCMV IEAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K83A
Plasmid#58401PurposeExpresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 83 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM3-K24A
Plasmid#58400PurposeExpresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cellsDepositorInsertIFITM3 (Ifitm3 Mouse)
UseTagsHAExpressionMammalianMutationLysine 24 to AlaninePromoterCMV IEAvailable sinceSept. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
ATF4 15: 5’ATF4.uORF1stopm1.GFP
Plasmid#21862DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStop codon for uORF1 converted to UCG (see author…PromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
FLAG-ELL2 pBacPAK8
Plasmid#15327DepositorInsertEleven Nineteen Lysine Rich in Leukemia 2 (ELL2 Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDNA CrkII Y221F
Plasmid#50732Purposemammalian expression of CrkII Y221F mutantDepositorInsertCrkII Y221F (CRK Human)
UseTagsExpressionMammalianMutationY221FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Jag1ICD-HA pBacPAK8-3
Plasmid#17331DepositorInsertJagged1 (Jag1 Rat)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only