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MSCV-6NBC-GFP-PGK-Puro-Barcode9

Plasmid

#83686

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode9

Use

Retroviral

Tags

Expression

Mutation

please view depositor comments below

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode7

Plasmid

#83684

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode7

Use

Retroviral

Tags

Expression

Mutation

L119P in GFP

Promoter

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-lin26

Plasmid

#80357

Purpose

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::lin-26 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-atg4.2

Plasmid

#80352

Purpose

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::atg4.2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 22, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-set6

Plasmid

#80362

Purpose

Pmex-5:GFP::H2B::PEST::set-6 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::set-6 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only
pCFJ150-ets4

Plasmid

#80355

Purpose

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::ets-4 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mex3

Plasmid

#80361

Purpose

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mex-3 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ150-mbk2

Plasmid

#80360

Purpose

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR reporter construct used for microinjection

Depositor

Sean Ryder

Article

Kaymak et al Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.

Insert

Pmex-5:GFP::H2B::PEST::mbk-2 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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ANKRD20A11P _pCSdest

Plasmid

#53837

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ANKRD20A11P (C21orf81 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
TMEM50B_pCSdest

Plasmid

#53835

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TMEM50B (TMEM50B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
DSCR8_pCSdest

Plasmid

#53828

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

DSCR8 (DSCR8 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SUMO3_pCSdest

Plasmid

#53947

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

SUMO3 (SUMO3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
S100B_pCSdest

Plasmid

#53895

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

S100B (S100B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
U2AF1_pCSdest

Plasmid

#53918

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

U2AF1 (U2AF1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SP6 and CMV

Available since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCRII-Topo Tmtc4

Plasmid

#45869

Depositor

Jeffrey Macklis

Article

Molyneaux et al J Neurosci. 2009 Sep 30;29(39):12343-54.

Insert

TMTC4 in situ probe (Tmtc4 Mouse)

Use

In situ

Tags

Expression

Mutation

nt 1220-1798 on BC031368

Promoter

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pBabe-HA-mMcl-1(T144D)

Plasmid

#25388

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(T144D) (Mcl1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

P142T T144D

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-mMcl-1(T144A)

Plasmid

#25384

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(T144A) (Mcl1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed threonine 144 to alanine

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA-mMcl1(S140A)

Plasmid

#25383

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1(S140A) (Mcl1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed serine 140 to alanine

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pMIG small T 88

Plasmid

#10691

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 88 (SV40gp7 simian virus)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

contains only the N-termial 88 amino acids

Promoter

Available since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
IA001: pMVP (L3-L2) 3x HA epitope tag+ polyA

Plasmid

#121750

Purpose

pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

3x HA epitope tag + polyA

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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