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Plasmid#84564PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK462-463-651RPromoternoneAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-rap1-K43-117-118R
Plasmid#84565PurposeThis plasmid can be used as a donor for subcloning into Gateway destination vectors.DepositorInsertRAP1 (RAP1 Budding Yeast)
UseGateway donor plasmidTagsExpressionMutationK43-117-118RPromoternoneAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoAC
Plasmid#100032PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with adenylyl cyclase activity.DepositorInsertRhoAC
UseTags1D4 (TETSQVAPA)ExpressionMammalianMutationE497K/C566DPromoterAdenovirus major late promoterAvailable sinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGS1212
Plasmid#63785PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection markerDepositorInserteGFP-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseGFP-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1DCH2AB
Plasmid#13472DepositorInsertMKP-1DCH2AB (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of MKP-1 CH2AB; contains aa 137-367PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 DN
Plasmid#58448Purposeexpression of drosophila YFP-Rab6 dominant negative mutantDepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationT26N (dominant negative)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST YFP Rab6 WT
Plasmid#58447Purposeexpression of drosophila YFP-Rab6DepositorInsertRab6 (Rab6 Fly)
UseTagsYFPExpressionInsectMutationPromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Kozak-rhTRIM5alpha-PFOS
Plasmid#79038PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertrhesus TRIM5alha-PFOS
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R53E/R59E/R61E
Plasmid#60355PurposeR53E R59E R61E triple mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR53E, R59E and R61E mutations; contains amino aci…PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWay21-MKP-1DCH2A
Plasmid#13470DepositorInsertMKP-1DCH2A (Dusp1 Mouse)
UseTagsEGFPExpressionMammalianMutationDeletion of MKP-1 CH2A; contains aa 47-367PromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_V5_LUC
Plasmid#45290DepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJune 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab1 DN
Plasmid#37682DepositorInsertRab1 (Rab1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS25N (dominant negative)PromoterGAL4Available sinceNov. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SH3BP4 W92A
Plasmid#46893Purposeexpresses HA tagged SH3BP4 W92A mutant in mammalian cellsDepositorInsertSH3BP4 W92A (SH3BP4 Human)
UseTagsHAExpressionMammalianMutationW92A (mutated SH3 domain)PromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab7 DN
Plasmid#37703DepositorInsertRab7 (Rab7 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT22N (dominant negative)PromoterGAL4Available sinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AS1371A
Plasmid#224409PurposeMammalian expression of KIF 13a S1371ADepositorInsertKIF 13a S1371A (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationS1371APromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF13AWLDLE
Plasmid#224410PurposeMammalian expression of KIF 13a WLDLEDepositorInsertKIF 13a WLDLE (Kif13a Mouse)
UseTagsGFPExpressionMammalianMutationWLDLEPromoterChicken Beta actinAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBa.M6PR-GFP
Plasmid#224407PurposeMammalian expression of M6PRDepositorInsertM6PR (M6PR Human)
UseTagsGFPExpressionMammalianMutationPromoterChicken Beta actinAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3109 (pSpy1C-Plac(1)_ffluc)
Plasmid#218514Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(1)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3111 (pSpy1C-Plac(3)_ffluc)
Plasmid#218778Purposereplicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressorDepositorInsertffluc
UseLuciferaseTagsExpressionBacterialMutationdeletion of mrfp cassette, mutagenesis of the RBS…PromoterPlac(3)Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA1
Plasmid#215398PurposeCm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)DepositorInsertcspA1
UseTags6xHis TagExpressionBacterialMutationCodon-optimized for E. coli BL21(DE3)PromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Spo20(PABD)
Plasmid#221758PurposeStable expression of GFP-Spo20(PABD) in mammalian cellsDepositorInsertSpo20 (SOS1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem Spo20(PABD) (amino acids 422-551)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TAPP1(PHD)
Plasmid#221757PurposeStable expression of GFP-TAPP1(PHD) in mammalian cellsDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TAPP1 (PHD) (amino acids 184-304)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PKD C1ab
Plasmid#221755PurposeStable expression of GFP-PKD C1ab in mammalian cellsDepositorInsertPrkd1(138-343) (Prkd1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 138-343PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA4114_Cterm_His
Plasmid#220484Purposeheterologous expression of PA4114 for protein purificationDepositorInsertPA4114
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA1472_Cterm_His
Plasmid#220485Purposeheterologous expression of PA1472 for protein purificationDepositorInsertPA1472
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_M15
Plasmid#220488Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiTagsExpressionMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-bacteria_speG_E23
Plasmid#220489Purposeinducible CRISPRiDepositorInsertgspeG
UseCRISPR and RNAiTagsExpressionMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speG_genomic
Plasmid#220473Purposeheterologous expression under endogenous speG E. coli promoterDepositorInsertspeG
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA4114
Plasmid#220474Purposeheterologous expression of PA4114 under endogenous speG E. coli promoterDepositorInsertPA4114
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1472
Plasmid#220475Purposeheterologous expression of PA1472 under endogenous speG E. coli promoterDepositorInsertPA1472
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_PA1377
Plasmid#220476Purposeheterologous expression of PA1377 under endogenous speG E. coli promoterDepositorInsertPA1377
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_speG_Nterm_His
Plasmid#220478Purposeheterologous expression for protein purificationDepositorInsertspeG
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA4114_Nterm_His
Plasmid#220479Purposeheterologous expression of PA4114 for protein purificationDepositorInsertPA4114
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA1472_Nterm_His
Plasmid#220480Purposeheterologous expression of PA1472 for protein purificationDepositorInsertPA1472
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA1377_Nterm_His
Plasmid#220481Purposeheterologous expression of PA1377 for protein purificationDepositorInsertPA1377
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_speG_Cterm_His
Plasmid#220483Purposeheterologous expression for protein purificationDepositorInsertspeG
UseTags6xHsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-PGAL-GFP-Cps1
Plasmid#207030PurposeExpression of GFP-Cps1 under GAL promoter.DepositorInsertCps1
UseTagsGFPExpressionYeastMutationPromoterGAL1Available sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast CG1358
Plasmid#218541PurposeDrosophila CG1358 cDNA (human codon optimized)DepositorInsertCG1358 (HisT Fly)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC5A7
Plasmid#218539Purposehuman SLC5A7 cDNADepositorInsertSLC5A7 (SLC5A7 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SLC44A1
Plasmid#218538Purposehuman SLC44A1 cDNADepositorInsertSLC44A1 (SLC44A1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lneg-INTR-GFP
Plasmid#208902Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius neglectus, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-BFP-P2A-ppViperin
Plasmid#217481PurposeExpression of Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRFL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-HFL1-8flag-LEU
Plasmid#207035PurposeExpression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).DepositorInsertHFL1
UseTags8xFLAGExpressionYeastMutationPromoterpHFL1Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-4V5-Pho8-Met
Plasmid#207029PurposeExpression of 4V5-Pho8 under CPU1 promoter.DepositorInsertPho8
UseTags4xV5ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp-PCUP1-4myc-Pho8
Plasmid#207028PurposeExpression of 4Myc-Pho8 under CPU1 promoter.DepositorInsertPho8
UseTags4xMycExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-Vac8-GFP-Ura
Plasmid#207027PurposeExpression of Vac8-GFP under CPU1 promoter.DepositorInsertVac8
UseTagsGFPExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-GFP-Vma13-Ura
Plasmid#207026PurposeExpression of GFP-Vma13 under CPU1 promoter.DepositorInsertVma13 ORF
UseTagsGFPExpressionYeastMutationPromoterpCUP1Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-mRuby3-Pho8-406
Plasmid#207043PurposeExpression of mRuby3-Pho8 under CPU1 promoter.DepositorInsertPHO8
UseTagsmRuby3ExpressionYeastMutationPromoterpCUP1Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-FLAG-HA-mmuMettl3 APPA
Plasmid#207124PurposeLentiviral plasmid to express mouse Mettl3 APPA mutantDepositorInsertMettl3 (Mettl3 Mouse)
UseLentiviralTagsFLAG-HAExpressionMutationD395A and W398APromoterSSFVAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only