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Plasmid#212950PurposeExpresses TALED_Left-Cox3.1-1397N. in mammalian cellsDepositorInsertTALED_Left-Cox3.1-1397N
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mTagBFP2-FRB-Rab7
Plasmid#214273PurposeMammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)DepositorArticleInsertmTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSCV_JAK3_IRES_BFP
Plasmid#206291PurposeExpresses the wild type form of human JAK3.DepositorInsertJAK3 (JAK3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWPI-LUC7L-3xFLAG
Plasmid#201640PurposeOver-expression of 3xFLAG-tagged human LUC7LDepositorInsertLUC7L (LUC7L Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-RRVQ
Plasmid#200188PurposeGateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editingDepositorInsertLbCas12a-RRVQ
UseCRISPR; Gateway compatible lbcas12a-rrv entry clo…TagsNLSExpressionPlantMutationG146R, D156R, R182V, E795QPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_1G11F2-3 light chain
Plasmid#198254Purposerecombinant expression of light chain of monoclonal anti-AMP antibody 1G11F2-3DepositorInsertlight chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse kappa)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-HaloGFP-Ca0.1(Na0.1, 143-144)
Plasmid#198599PurposeGreen fluorescent Ca2+ ion indicator for bacterial expression.DepositorInsertHaloGFP-143-144
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-2xHA
Plasmid#193718PurposeExpression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-2xHA (KIF5B Synthetic, Human)
UseTags2xHAExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3X-UASTattB
Plasmid#189859PurposeFly cloning and transformationDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pST_DSUP_RAMVA (pBS0353)
Plasmid#185189PurposeFor the mammalian expression of the tardigrade protein DSUP_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertDSUP_RAMVA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(WT)
Plasmid#182659PurposeExpresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertwild type mouse neurofilament light chain (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-RIIα
Plasmid#168492PurposeMammalian expression of mouse PKA regulatory subunit Iiα, untagged.DepositorInsertmouse PKA regulatory subunit IIα, untagged
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWL89A mmPing20
Plasmid#145788PurposemPing Yeast Transposition AssayDepositorInsertmmPing20
UseTagsExpressionYeastMutationT162C, T258A, T287C, T300A, T304A, T310A, G372APromoterAvailable sinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
PHKG1 gRNA (BRDN0001144981)
Plasmid#76356Purpose3rd generation lentiviral gRNA plasmid targeting human PHKG1DepositorInsertPHKG1 (PHKG1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF3482
Plasmid#144958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX2 (SOX2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
ER-B
Plasmid#209870PurposeDimerization dependent fluorescent protein B anchored to cytosolic face of the endoplasmic reticulum membrane with N-terminal targeting sequence of rabbit CYP2C1DepositorInsertddGFP B
UseTagsTargeting domain of CYP2C1 MDPVVVLGLCLSCLLLLSLWKQ…ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX313-Firefly luciferase
Plasmid#118017PurposeConstitutive expression of Firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-mCherry
Plasmid#45147DepositorInsertLAMP1-mCherry (LAMP1 Synthetic, Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT
Plasmid#1771PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybSEP2-WPRE
Plasmid#207661PurposeMonitoring neuropeptide releaseDepositorInsertCytochrome b-561 (Cyb561 Mouse)
UseAAVTagsExpressionMutationHistidine 86 and 159 to alanines, inserted 2 X SE…PromoterhSynapsinAvailable sinceDec. 5, 2023AvailabilityAcademic Institutions and Nonprofits only