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Plasmid#118863Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C proteaseDepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE656
Plasmid#153254PurposeM2 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE474
Plasmid#153265PurposeM1 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE475
Plasmid#153266PurposeM2 shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEG R2-L3 #BGV193
Plasmid#48952PurposeFor inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-PpU6P-sgRNA-L1R5
Plasmid#113737PurposeGateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.DepositorInsertPpU6P::sgRNA
UseCRISPR; Gateway entry vectorTagsExpressionMutationPromoterP. patens U6 promoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTE105
Plasmid#59400PurposeMethyloBrick expression vector, tuf promoter; TcRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtufAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC7
Plasmid#118876Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT346_pLambda-attB-pCA-GFP-pA
Plasmid#52551Purposefor producing PhiC31 attB-containing minicircle in vitroDepositorInsertsLambda integrase attL1
Lambda integrase attR1
PhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTE101
Plasmid#59396PurposeMethyloBrick cloning vector, no promoter; KmRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoternoneAvailable sinceDec. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOCC154
Plasmid#118902Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
UseTagsTwinStrep, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERT-myc
Plasmid#107456PurposeMammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2DepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA-gRic6T
Plasmid#106401PurposeE.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoterpj23119Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEINO4
Plasmid#179488PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertaadA1
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC153
Plasmid#118901Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3CDepositorInsertNcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsTwinStrep, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEINO3
Plasmid#179487PurposeMulti-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugationDepositorInsertTetR/A
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L1-dMb2Cas12a
Plasmid#177683PurposeGateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editingDepositorInsertFokI-dMb2Cas12a
UseCRISPR; Gateway compatible foki-dmb2cas12a entry …TagsNLSExpressionPlantMutationPromoterAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only