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Addgene
Showing: 2481 - 2500 of 2808 results

  1. pCVD438

    Plasmid
    #63759
    Purpose
    eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pNR64

    Plasmid
    #79540
    Purpose
    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
    Depositor
    Timothy Lu
    Article
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    Use
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79540-plasmid-map-sequence-id-148741

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  8. pHBJT003

    Plasmid
    #225154
    Purpose
    Medium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.
    Depositor
    Jenny-Lee Thomassin
    Article
    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    FLAG epitope tag (35 bp)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225154-plasmid-map-sequence-id-444670

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  14. pSin4 UAP1 F383G

    Plasmid
    #169891
    Purpose
    Used for generation of stable mammalian cell lines expressing UAP1 with with F383G mutation
    Depositor
    Jennifer Kohler
    Article
    Capota et al Curr Protoc. 2021 Jul;1(7):e201. doi: 10
    Insert
    UDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
    Use
    Lentiviral
    Tags
    None
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine (F) at position 383 to glyc…
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169891-plasmid-map-sequence-id-333152

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  15. pCL66-decoder

    Plasmid
    #159453
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. Same insert as pCL33-decoder in a multiple integration shuttle vector. Backbone: pRG235 (addgene). Marker: Leu2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pfus1mut
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159453-plasmid-map-sequence-id-313208

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Showing: 2481 - 2500 of 2808 results