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Showing: 2481 - 2500 of 72238 results

  2. pLyGo-Ec-6

    Plasmid
    #163132
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163132-plasmid-map-sequence-id-338662

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  6. Barcode backbone

    Plasmid
    #158665
    Purpose
    This plasmid serves as the backbone for the visual barcodes described in our paper. It has a CMV-Puro-F2A backbone to which we insert a fluorescent protein cellular localization combination
    Depositor
    Ravid Straussman
    Article
    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    hcRED
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    158665-plasmid-map-sequence-id-309764

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  10. pBP-ORF

    Plasmid
    #72949
    Purpose
    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Level 0 - ORFs
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    CAT gene - C435G (nucleotide) - silent mutagenesi…
    Promoter
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72949-plasmid-map-sequence-id-140106

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  11. pAGW-dpse-GAL4-DBD

    Plasmid
    #125153
    Purpose
    Gateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CP
    Depositor
    Alexander Stark
    Article
    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:
    Insert
    D. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
    Use
    Gateway fly expression vector
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    125153-plasmid-map-sequence-id-248016

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  13. SMT205

    Plasmid
    #134817
    Purpose
    SMT201 with mutated RBS for DHFR, in vivo assays
    Depositor
    Tanja Kortemme
    Article
    Thompson et al Elife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134817-plasmid-map-sequence-id-274011

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  14. pAL950

    Plasmid
    #119748
    Purpose
    precursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Depositor
    Linda Randall
    Article
    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1
    Insert
    precusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Internal NdeI site removed and mutations Alanine …
    Promoter
    T7
    Available since
    Jan. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    119748-plasmid-map-sequence-id-232481

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  18. pDest-pol2-Smarcd1-Myc

    Plasmid
    #227716
    Purpose
    Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.
    Depositor
    Kent Hunter
    Article
    Ross et al bioRxiv 2024.01.24.577061
    Insert
    Smarcd1 (Smarcd1 Mouse)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    pol2
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227716-plasmid-map-sequence-id-450967

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  19. 3xFLAG-dCas9/pMSCVpuro

    Plasmid
    #134983
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    April 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    134983-plasmid-map-sequence-id-430623

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Showing: 2481 - 2500 of 72238 results