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Plasmid#206439PurposeCCR4 expression in Schneider cells for imagingDepositorInsertCCR4 (twin Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-NOT2 isoform A (HK54)
Plasmid#206441PurposeNOT1 expression in Schneider cells for imagingDepositorInsertNOT1 (Not1 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-mEGFP-NOT3 isoform A (HK55)
Plasmid#206442PurposeNOT3 expression in Schneider cells for imagingDepositorInsertNOT3 (Not3 Fly)
UseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-NOT2 isoform A (HK99)
Plasmid#206456PurposeNOT2 expression in Schneider cells for imagingDepositorInsertNOT2 (Rga Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Oskar 241-387 (DR) (HK74)
Plasmid#206462PurposeOskar 240-387 expression in Schneider cells for imagingDepositorInsertOskar 240-387 (osk Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-Bam isoform A (HK62)
Plasmid#206466PurposeBam expression in Schneider cells for imagingDepositorInsertBam (bam Fly)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-CCR4 isoform A-mCherry-PH (EB7)
Plasmid#206473PurposeCCR4 expression in Schneider cells for imagingDepositorInsertCCR4 (twin Fly)
UseTagsmcherry PHExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-OST4-mCherry-CAF1 isoform A (HK184)
Plasmid#206476PurposeCAF1 expression in Schneider cells for imagingDepositorInsertCAF1 (Pop2 Fly)
UseTagsOST4 mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET16_Caspase-4_p10
Plasmid#214308PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT
Plasmid#214075PurposeLuminescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRT_RTctrl
Plasmid#214078PurposeLuminescent reporter system, readthrough control, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTi
Plasmid#214076PurposeLuminescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationLinker sequence present between firefly luciferas…PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LiRTX_ctrl
Plasmid#214077PurposeLuminescent reporter system, NMD negative control. Intron present in 5'UTRDepositorInsertFirefly luciferase
UseTagsExpressionMammalianMutationRemnant of linker sequence present at the end of …PromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-11_humanized-mutant
Plasmid#214315PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Mouse)
UseTagsHis-TEVExpressionBacterialMutationN152K_KLS288YKT_KASIHS348TPRAKAPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Caspase-11_rat_catalytic domain
Plasmid#214329PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (Casp4 Rat)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_W267N
Plasmid#214314PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationW267NPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_R269D
Plasmid#214313PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationR269DPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_K356D
Plasmid#214312PurposeProtein overexpression in bacteriaDepositorInsertCASP4 (CASP4 Human)
UseTagsHis-TEVExpressionBacterialMutationK356DPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-5-enzymatic-domain
Plasmid#214306PurposeProtein overexpression in bacteriaDepositorInsertCASP5 (CASP5 Human)
UseTagsHis-TEVExpressionBacterialMutationaa133-434PromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_Pro-IL-18_lemur
Plasmid#214332PurposeProtein overexpression in bacteriaDepositorInsertIL18
UseTagsHis-TEV and MycExpressionBacterialMutationPromoterT7Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only