-
Plasmid#153310PurposeTet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GWDepositorInsertTetR
UseTransposonTagsExpressionMutationPromoterSV40Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
CVB3 IRES
Plasmid#189968PurposePart 2 plasmid to introduce the CVB3 IRES into circRNAsDepositorInsertCVB3 IRES
UseCloningTagsExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-INU-FLAG-FAM134Bs
Plasmid#128261PurposeExpresses FAM134Bs in mammalian cellsDepositorInsertFAM134Bs (RETREG1 Human)
UseRetroviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0915 (CRISPR with target 3)
Plasmid#130643PurposeExpresses CRISPR RNA from a T7 promoter for VchCAST system. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertCRISPR(target-3)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterT7Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-EBFP2-NLS (pRW1447)
Plasmid#216219PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expressionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_StrepII_TEV_PP2Ac
Plasmid#217477PurposeExpresses PP2Ac with a TEV cleavable N-terminal StrepII tagDepositorInsertPP2Ac
UseTagsStrepII, TEVExpressionMammalianMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMycDam
Plasmid#59218PurposeDrosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli DamDepositorTypeEmpty backboneUseDamidTagsDam and MycExpressionInsectMutationPromoterDrosophila heat-shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-BDNF-pHluorin
Plasmid#187193PurposeTo express the mouse BDNF tagged with the FP pHluorinDepositorInsertBDNF (Bdnf Mouse)
UseLentiviralTagspPHluorinExpressionMammalianMutationThe codon were optimized for better expressionPromoterCMVAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.eGFP.WPRE.hGH
Plasmid#105549PurposeAAV expression of EGFP from GFAP promoterDepositorHas ServiceAAV5InserteGFP
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-Jx
Plasmid#51905PurposeCre-dependent On/Off aav vectorDepositorTypeEmpty backboneUseAAVTagsExpressionMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2792
Plasmid#143193PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF282 (ZNF282 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBSDONR P4r-P2
Plasmid#121527PurposeGateway Entry Vector for Multisite Gateway CloningDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHARGE3
Plasmid#24658DepositorInsertTurbo
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2010AvailabilityAcademic Institutions and Nonprofits only -
NC16 pCDNA3.1 FLAG NRF2
Plasmid#36971DepositorInsertNRF2 (NFE2L2 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
negMaLionB
Plasmid#113911Purposenegative control for Blue ATP sensorDepositorInsertnegMaLionB
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGCG26
Plasmid#82371PurposeYFP expressed from pJ23119 in the glpK locus with gentamicin resistanceDepositorInsertYFP
UseTagsExpressionMutationPromoterPJ23119Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
pmGFP-ADAR1-p150
Plasmid#117927PurposeADAR1-p150 expression plasmid.DepositorInsertADAR1-p150 (ADAR Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXR004: CasRx pre-gRNA cloning backbone
Plasmid#109054PurposehU6-driven expression of guide RNAs compatible with CasRx. Contains BbsI sites for guide cloning flanked by 5' and 3' full-length DRsDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-sgRNA-RFP
Plasmid#166005PurposeBacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cytoplasmic PancACe
Plasmid#215708PurposeExpresses acetyl-CoA biosensor in the cytoplasmDepositorInsertPancACe
UseLentiviralTagsFLAG, HA, and cytoplasmic localization sequenceExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_Flag_TP53_WT
Plasmid#183112PurposeExpresses flag-tagged p53 in mammalian cellsDepositorInsertTP53 (TP53 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Mesp1
Plasmid#72687PurposeDoxycyline regulated expression of Mesp1 in mammalian cellsDepositorInsertMesp 1 (MESP1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
YCe3736_HC_Amp_ccdB_receiver vector
Plasmid#100637PurposeEMMA toolkit receiver vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pAGM35171
Plasmid#153222PurposeLevel 1 position 1 part containing the Nos promoter, the BAR gene, and the Ocs terminatorDepositorInsertNos promoter, BAR gene, Ocs terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
pCMV-8xHis-Ub
Plasmid#107392PurposeMammalian expression 8xHis-UbDepositorInsert8 tandem repeats of 6xHis-Ub (See comments below)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS26
Plasmid#32295DepositorTypeEmpty backboneUseBacterial gene additionTagsExpressionBacterialMutationPromoterrhaBAvailable sinceSept. 26, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKG96
Plasmid#63072PurposeRetroviral expression of EGFP-TEV-S-tag CENP-A for protein localization and affinity purification (LAP)DepositorInsertCENP-A (CENPA Human)
UseRetroviralTagsEGFP and TEV-SExpressionMammalianMutationA12V, C75Y, and L121F mutations compared to GenBa…PromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
hCCR5-LSSmOrange
Plasmid#110194PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FPDepositorInserthCCR5 (CCR5 Human)
UseTagsLSSmOrangeExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
mPA-GFP-H2B-6
Plasmid#57137PurposeLocalization: Nucleus/Histones, Excitation: 400 / 504, Emission: 515 / 517DepositorInsertH2B (H2BC11 Human)
UseTagsmPA-GFPExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hs.HRAS Q61R
Plasmid#83188PurposeGateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutationDepositorInsertHRAS (HRAS Human)
UseGateway entry cloneTagsExpressionMutationQ61RPromoterAvailable sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-hCOL1A1
Plasmid#140112PurposeExpresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage siteDepositorInsertType I procollagen α1 (COL1A1 Human)
UseTagsmCeruleanExpressionMammalianMutationCOL1A1 exons 2-3 replaced by fluorescent tagPromoterCMVAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
ERN1 gRNA (BRDN0001149471)
Plasmid#76410Purpose3rd generation lentiviral gRNA plasmid targeting human ERN1DepositorInsertERN1 (ERN1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mVenus-ATG16-N-18
Plasmid#56323PurposeLocalization: Membrane Receptor, Excitation: 515, Emission: 528DepositorInsertATG16
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTF101.1
Plasmid#134770PurposeA binary plasmid containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorArticleTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only