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Showing: 3721 - 3750 of 4144 results

  2. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

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  3. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

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  4. pTH858-gst-optec V156E

    Plasmid
    #89592
    Purpose
    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed valine 156 to glutamic acid
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89592-plasmid-map-sequence-id-206041

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  5. pTH859-gst-optec Y172M

    Plasmid
    #89593
    Purpose
    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    Use
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed tyrosine 172 to methionine
    Promoter
    T7
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89593-plasmid-map-sequence-id-206040

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  9. pJK278

    Plasmid
    #71134
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to asparagine; additional N-…
    Promoter
    T7
    Available since
    Feb. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    71134-plasmid-map-sequence-id-131033

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  10. pJK130

    Plasmid
    #71129
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed valine-319 to phenylalanine
    Promoter
    T7
    Available since
    Feb. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    71129-plasmid-map-sequence-id-131030

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  11. pJK591

    Plasmid
    #71589
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala/Asp177>Ala mutants (UctC-T2A,D177A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al PLoS One. 2013 Jul 23;8(7):e67901. doi:
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine and aspartate-177 …
    Promoter
    T7
    Available since
    Jan. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    71589-plasmid-map-sequence-id-130927

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  14. pJK320

    Plasmid
    #71144
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59S mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to serine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71144-plasmid-map-sequence-id-131043

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  15. pJK319

    Plasmid
    #71143
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59A mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to alanine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71143-plasmid-map-sequence-id-131042

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  16. pJK318

    Plasmid
    #71142
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59Q mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to glutamine; additional N-t…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71142-plasmid-map-sequence-id-131041

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  17. pJK315

    Plasmid
    #71141
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89G mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to glycine; additional N-ter…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71141-plasmid-map-sequence-id-131040

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  18. pJK314

    Plasmid
    #71140
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to valine; additional N-term…
    Promoter
    T7
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71140-plasmid-map-sequence-id-131039

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  19. pJK174

    Plasmid
    #71835
    Purpose
    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7 and endogenous
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71835-plasmid-map-sequence-id-131052

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  20. pJK279

    Plasmid
    #71135
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71135-plasmid-map-sequence-id-131034

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  21. pJK283

    Plasmid
    #71139
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59N mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine; additional N-…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71139-plasmid-map-sequence-id-131038

    Enlarge

  22. pJK282

    Plasmid
    #71138
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59D mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate; additional N-t…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71138-plasmid-map-sequence-id-131037

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  23. pJK281

    Plasmid
    #71137
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71137-plasmid-map-sequence-id-131036

    Enlarge

  24. pJK280

    Plasmid
    #71136
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to phenylalanine; additional…
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71136-plasmid-map-sequence-id-131035

    Enlarge

  25. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

    Enlarge

  26. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  27. pET:CACT:GFP

    Plasmid
    #60737
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60737-plasmid-map-sequence-id-97723

    Enlarge

  29. Fast-GCaMP-RS09

    Plasmid
    #52649
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS09
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L414T mutated from GCaMP3
    Promoter
    T7
    Available since
    May 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  30. Fast-GCaMP-RS05

    Plasmid
    #52646
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS05
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M373Q mutated from GCaMP3
    Promoter
    T7
    Available since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3721 - 3750 of 4144 results