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Plasmid#21654PurposeRetroviral backbone for cloning and gene expression. Select with puromycin or GFP.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsGFP and IRESExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only
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pDEST-cuOx144_Bxb1attB_loxP-PGKpuro-loxP
Plasmid#119903PurposeBxb1 integration plasmid for cuO array with Puro selection cassetteDepositorInsertcuOx144 array
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
InsRA-eGFP
Plasmid#79795PurposeInsulin receptor isoform A with a inter-domain eGFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.DepositorInsertInsRA (INSR Human)
UseTagseGFP extracellularExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP
Plasmid#188778PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiFC-VN173
Plasmid#22010PurposeMammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assaysDepositorInsertVN173
UseTagsFLAGExpressionMammalianMutationVenus residus 1-172PromoterAvailable sinceSept. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-TLR7-YFP
Plasmid#13022DepositorInsertTLR7 (TLR7 Human)
UseTagsYFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-Linker-GFP
Plasmid#211734PurposeExpresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEx-ER-TurboID
Plasmid#160857PurposeCre recombinase-dependent expression of ER TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selectionDepositorInsertER TurboID
UseAAVTagsHA and V5ExpressionMutationPromoterCAGAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Sp-pegRNA-RNF2_+5GtoT
Plasmid#135957PurposeS. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editingDepositorInsertRNF2_5GtoT pegRNA
UseTagsExpressionMammalianMutationSee manuscriptPromoterU6Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only