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HIV-dual-GT

Plasmid

#122696

Purpose

Dual-fluorescence reporter to monitor the expression of HIV-1 early genes and late genes simultaneously.

Depositor

Klaus Überla

Article

Xiao et al mBio. 2021 Jan 19;12(1). pii: mBio.02525

Inserts

mTagBFP2
DsRed-max

Use

Lentiviral

Tags

PEST domain

Expression

Mutation

Promoter

HIV-1 LTR

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-FKBP:mCherry-Rab5a

Plasmid

#72901

Purpose

Early endosome-targeted mCherry-tagged recruiter construct

Depositor

Ivan Yudushkin

Article

Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

Insert

Rab5a (RAB5A Human)

Use

Lentiviral

Tags

FKBP(F36V) and mCherry

Expression

Mammalian

Mutation

fragment a.a. 2-215

Promoter

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Article

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLL3.7-EF-EYFP-YAP1_WT-PolyA

Plasmid

#112284

Purpose

Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

Depositor

Erik Sahai

Article

Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

Insert

EYFP-YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT human YAP1-2gamma

Promoter

EF-1 alpha

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-117G-hyA3A-BE4max

Plasmid

#157945

Purpose

Lentiviral expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

Lenti-117G- hyA3A-BE4max

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-tdTomato-FlagAkt1W80A

Plasmid

#64833

Purpose

cDNA of Akt1 with point mutation from Trp to Ala at position 80. This mutation confers resistance to the MK-2206 drug

Depositor

Eva Gonzalez

Article

Kajno et al Biochem J. 2015 Apr 9.

Insert

Akt1 (Akt1 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Point mutation from Trp to Ala at position 80

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only
Fuw-dCas9-Dnmt3a-P2A-tagBFP

Plasmid

#84569

Purpose

Lentiviral construct to express dCas9 fused with Dnmt3a-P2A-tagBFP

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

dCas9-Dnmt3a (DNMT3A Synthetic, Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_mTurquoise2

Plasmid

#113895

Purpose

Mammalian (inducible) expression of cytosolic mTurquoise2

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

monomeric Turquoise2

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV-MIE-TetO2

Available since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-BSD-tet-mEGFP-MIB1

Plasmid

#140241

Purpose

Lentiviral vector expressing mEGFP-tagged MIB1 upon tetracycline treatment

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Tet-inducible mEGFP-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBITE-EGFRvIII-F263-OKT3

Plasmid

#190680

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F263 clone)

Depositor

Scott McComb

Article

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F263-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-RNaseHI-NES-EGFP

Plasmid

#196701

Purpose

Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

bacterial RNaseHI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

Plasmid

#141392

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf9b

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-tetO-Tfap2c

Plasmid

#70269

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

transcription factor AP-2, gamma (Tfap2c Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER20_mGFP

Plasmid

#190027

Purpose

Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal mGFP

Depositor

Andrew Tutt

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pKH159_FLAG-APEX2-YIPF4 WT

Plasmid

#214978

Purpose

Flag-APEX2 tagged YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF4 (YIPF4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH162_FLAG-APEX2-YIPF3 LIR mut

Plasmid

#214981

Purpose

Flag-APEX2 tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH143_EGFP-YIPF3 LIR mut

Plasmid

#214975

Purpose

GFP tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

LIR mutant

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH142_EGFP-YIPF3 WT

Plasmid

#214974

Purpose

GFP tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

GFP-YIPF3 (YIPF3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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