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Plasmid#78259PurposepsiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertmutated miR-34 target site
UseLuciferase; Microrna activityTagsExpressionMammalianMutationnucleotides 2-5 and 10-11 changed from CCGT to GG…PromoterAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_PHC3
Plasmid#45262DepositorInsertPHC3 (PHC3 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH6 - pBait-ChiX
Plasmid#174662Purposearabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertChiX
UseTags1xMS2 hairpinExpressionBacterialMutationPromoterpBadAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAPcrRNA_Lmo
Plasmid#126501PurposeL.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
linker-mCitrine-PIF6-NAT
Plasmid#51576PurposeTag protein of interest with PIF6 in Saccharomyces cerevisiaeDepositorInsertPIF6 (PIL2 Mustard Weed)
UseTagslinker and mCitrineExpressionYeastMutation1-100aa of PIF6PromoternoneAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CFP-GAI(1-532)
Plasmid#37310DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFPExpressionMammalianMutationcontains aa 1-532PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFKBPmCherry-Akt2(K14A)
Plasmid#86597PurposeExpresses recruitable mCherry-tagged human Akt2(K14A) membrane-binding mutantDepositorInsertFKBB-mCherry-Akt2(K14A) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)
Plasmid#86595PurposeExpresses eGFP-tagged human Akt2(K14A/R25E) membrane-binding mutantDepositorInsertAkt2(K14A/R25E) (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lyn-mCherry-GAI(1-92)
Plasmid#37315DepositorInsertGAI (GAI Mustard Weed)
UseTagsLyn and mCherryExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lyn-CFP-GAI(1-92)
Plasmid#37311DepositorInsertGAI (GAI Mustard Weed)
UseTagsCFP and LynExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pZ005 (AacC2c1 human codon optimized protein)
Plasmid#70171PurposeExpresses AacC2c1 human codon optimized proteinDepositorInsertAacC2c1 protein
UseTagsNLSExpressionMutationPromoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIND-CLucZ
Plasmid#53224PurposeInducible expression of Cypridina luciferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-Akt2(T309A)
Plasmid#86625PurposeExpresses eGFP-tagged human Akt2(T309A) phosphomutantDepositorInsertAkt2(T309A) (AKT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
C1-F-tractin-mCitrine
Plasmid#155217PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)
UseTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLAC
Plasmid#45072Purposelacritin-intein fusion plasmid coding for intact mature lacritin without signal peptideDepositorInsertLacritin (LACRT Human)
UseProtein expression vectorTagsInteinExpressionBacterialMutationPromoterT7Available sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc NES-mCE NLS
Plasmid#82466PurposeExpresses cytoplasmic restricted form of myc tagged mRNA capping enzymeDepositorInsertNES mRNA capping enzyme (Rngtt Mouse, HIV)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-GAI(1-92)
Plasmid#37317DepositorInsertGAI (GAI Mustard Weed)
UseTagsNLSExpressionMammalianMutationcontains aa 1-92PromoterCMVAvailable sinceJune 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_SLC2A2
Plasmid#45267DepositorInsertSLC2A2 (SLC2A2 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
AQTFAATHNPWASQA_epitope
Plasmid#165019PurposeExpression of CMV UL86 (1222-1236) fused to CD74DepositorInsertCMV UL86 (1222-1236)
UseLentiviralTagsCD74 at N-term; cathepsin S cleavage site (GRWHTV…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only