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pBABE puro K-Ras V12

Plasmid

#9052

Purpose

Retroviral expression of a constitutively active form of K-Ras

Depositor

William Hahn

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

G12V. Constitutively active.

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pCW-eGFP

Plasmid

#162823

Purpose

Doxycycline-inducible overexpression of eGFP

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

V163A

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-12S-E1A-HA-puro

Plasmid

#212671

Purpose

Express adenovirus E1A

Depositor

Tobias Meyer, Yumi Konagaya

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

12S-E1A

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

TRE

Available since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-EGFP

Plasmid

#122848

Purpose

N-terminal EGFP tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Article

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pTRE Tet ON

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-mCherry

Plasmid

#123221

Purpose

N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Nuclear PancACe

Plasmid

#215706

Purpose

Expresses acetyl-CoA biosensor in the nucleus

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025. 10.1016/j.chembiol

Insert

PancACe

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-Kras G12D

Plasmid

#58902

Purpose

Expresses human Kras with a G12D Mutation

Depositor

Channing Der

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

G12D mutation

Promoter

Available since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only

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CAG-FR-GECO1c

Plasmid

#163682

Purpose

Far-red fluorescent calcium indicator FR-GECO1c

Depositor

Robert Campbell

Article

Dalangin et al bioRxiv 2020.11.12.380089

Insert

FR-GECO1c

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-Blasticidin

Plasmid

#75085

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti UBC hNLRP3-mNG

Plasmid

#218642

Purpose

Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (NLRP3 Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pMK444 (EF1-OsTIR1(F74G) Puro)

Plasmid

#214374

Purpose

EF1-OsTIR1(F74G) (PiggyBac)

Depositor

Masato Kanemaki

Article

Hatoyama et al EMBO Rep. 2024 Aug 23. doi: 10.1038/s443

Insert

EF1-OsTIR1(F74G)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1 promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pLVpuro-CMV-N-3xFLAG

Plasmid

#123223

Purpose

N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Article

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-MARK1_IDG-K

Plasmid

#170582

Purpose

Gateway destination clone of MARK1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-MARK1 (MARK1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLJM1 flag-cFLIP

Plasmid

#211529

Purpose

Expresses flag-tagged cFLIP (CFLAR)

Depositor

Richard Possemato

Insert

cFLIP (CFLAR Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-MPAct-mRuby3-IRES-Puro

Plasmid

#155231

Purpose

lentiviral MPAct construct

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

Lentiviral expression of MPAct-mRuby sensor

Use

Lentiviral

Tags

mRuby3

Expression

Mutation

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-p62

Plasmid

#38277

Depositor

Noboru Mizushima

Article

Itakura et al J Cell Biol. 2011 Jan 10;192(1):17-27.

Insert

Sequestosome-1 (Sqstm1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2012

Availability

Academic Institutions and Nonprofits only

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