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Showing: 2461 - 2480 of 13761 results

  1. pET28a HAT epitope

    Plasmid
    #129445
    Purpose
    expression of HAT epitope
    Depositor
    Oskar Laur
    Insert
    HAT epitope
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129445-plasmid-map-sequence-id-257459

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  2. pET-FLAG-B-eSpCas9

    Plasmid
    #126772
    Purpose
    Expression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    T7
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126772-plasmid-map-sequence-id-410600

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  8. pET14b-dabAB2 D353A

    Plasmid
    #133016
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a D353A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    D353A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133016-plasmid-map-sequence-id-262984

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  9. pET14b-dabAB2 C351A

    Plasmid
    #133014
    Purpose
    pET14b carrying the dabA2 gene (Uniprot: D0KWS7)  with a C351A mutation fused to a c-terminal strep tag and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag
    Depositor
    David Savage
    Article
    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038
    Inserts
    dabB2
    dabA2
    Use
    Tags
    6xHis, StrepII tag, and sfGFP
    Expression
    Bacterial
    Mutation
    C351A
    Promoter
    t7
    Available since
    Nov. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    133014-plasmid-map-sequence-id-262980

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Showing: 2461 - 2480 of 13761 results