Addgene

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M_NLS 34kDa

Plasmid

#201339

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-1PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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M_NLS 47kDa

Plasmid

#201343

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-3PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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H_NLS 41kDa

Plasmid

#201340

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-nCLC

Plasmid

#193564

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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nCLC-ShadowY

Plasmid

#193565

Purpose

Expresses human Clathrin Light Chain B (neuronal isoform) tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Neuronal isoform

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-CLC

Plasmid

#193567

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

FKBP and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CLC-FKBP-EGFP

Plasmid

#193572

Purpose

Expresses human Clathrin Light Chain B tagged with FKBP and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

FKBP and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-CLC-SNAP

Plasmid

#193580

Purpose

Expresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

SNAP-tag and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-ShadowY-CLC

Plasmid

#193552

Purpose

Expresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain A (Clta Mouse)

Use

Tags

ShadowY and mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
ShadowY-CLC

Plasmid

#193560

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
CLC-ShadowY

Plasmid

#193561

Purpose

Expresses human Clathrin Light Chain B tagged with ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Use

Tags

ShadowY

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
eDrMet

Plasmid

#183999

Purpose

Optically controlled eDrcMet expression in mammalian cells

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrMet (MET Human)

Use

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D

Plasmid

#176462

Purpose

expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-Ub-VPS13D N3521S

Plasmid

#176463

Purpose

expressing VPS13D patient mutant N3521S in mammalian cells

Depositor

Richard Youle

Article

Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

VPS13D (VPS13D Human)

Use

Tags

GFP-Ub

Expression

Mammalian

Mutation

N3521S mutation

Promoter

CMV

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-Cdc42-G12V-DeltaCaaX

Plasmid

#176121

Purpose

Constitutivley active, mitochondrial localized Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

CDC42 (CDC42 Human)

Use

Tags

Expression

Mammalian

Mutation

G12V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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TOMM20-mTurquoise-RhoA-G14V-deltaCaaX

Plasmid

#176123

Purpose

Constitutively active, mitochondrial localized RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOA (RHOA Human)

Use

Tags

Expression

Mammalian

Mutation

G14V

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-CD8a(7A)

Plasmid

#162498

Purpose

Expresses GFP tagged CD8a mutant in mammalian cells. The seven O-glycosylation sites in its stem region are mutated.

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

CD8a with a mutated stem region (CD8A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

The five threonine and two serine residues are mu…

Promoter

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (184-618)

Plasmid

#127626

Purpose

Human Miro1 lacking the first GTPase domain

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

184-618 only

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Miro1 (412-618)

Plasmid

#127628

Purpose

GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

412-618 only

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BtGRK2[D110A K220R]-mVenus

Plasmid

#137779

Purpose

Visualization of GRK2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Bovine GRK2[D110A K220R] (GRK2 Bovine)

Use

Tags

Expression

Mammalian

Mutation

D110A K220R

Promoter

CMV

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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