Skip to main content
Addgene
Showing: 2461 - 2480 of 35252 results

  1. pSilencer2.1-U6-3JB1F+2

    Plasmid
    #190833
    Purpose
    For mammalian expression of 3JB1F+2, a dual-specificity aptamer that binds OGT and GFP. Expression is driven by a U6 promoter. This dual-specificity aptamer adopts a folded linker.
    Depositor
    Gerald Hart
    Article
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    3JB1F_T1+2bp (A Dual-Specificity aptamer targeting OGT and GFP)
    Use
    Affinity Reagent/ Antibody and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190833-plasmid-map-sequence-id-379189

    Enlarge

  4. psiCHECK2-miR-34 MT

    Plasmid
    #78259
    Purpose
    psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene
    Depositor
    Joanne Weidhaas
    Article
    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10
    Insert
    mutated miR-34 target site
    Use
    Luciferase; Microrna activity
    Tags
    Expression
    Mammalian
    Mutation
    nucleotides 2-5 and 10-11 changed from CCGT to GG…
    Promoter
    Available since
    June 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    78259-plasmid-map-sequence-id-149444

    Enlarge

  17. pBAD-tdTEVDB_C-15A/G4C

    Plasmid
    #177205
    Purpose
    Permuted td intron with evolved initiation sequence for the expression of circular mRNA
    Depositor
    Stephen Fried
    Article
    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175
    Insert
    Permuted GFP with evolved initiation sequence for circular mRNA
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Permuted sfGFP after residue 52 & Changed Cyt…
    Promoter
    araBAD
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    177205-plasmid-map-sequence-id-373215

    Enlarge

  20. TF-bio-His

    Plasmid
    #52184
    Purpose
    Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TF (TF Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52184-plasmid-map-sequence-id-80548

    Enlarge

Showing: 2461 - 2480 of 35252 results