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pRK5-HA-Sestrin2

Plasmid

#72593

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN2 (SESN2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pF420

Plasmid

#123659

Purpose

Produces cofactor F420 in E. coli

Depositor

Colin Jackson

Article

Bashiri et al Nat Commun. 2019 Apr 5;10(1):1558. doi:

Inserts

CofD
FbiB
FbiD
FbiC

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

BBa_R0040

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pBS L30 Ruby3-Rab6A

Plasmid

#135653

Purpose

Rab6A fused to the red fluorescent protein Ruby3 in N-ter under control of a weak L30 promoter.

Depositor

Raphael Gaudin

Article

Deffieu et al Sci Adv. 2021 Jan 8;7(2). pii: 7/2/eaba7

Insert

Rab6A

Use

Tags

Ruby3

Expression

Mammalian

Mutation

Promoter

L30

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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PRPF18

Plasmid

#155846

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF18 (PRPF18 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 DN

Plasmid

#13051

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Use

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); aspara…

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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THOC3

Plasmid

#156082

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC3 (THOC3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-hcRED

Plasmid

#115440

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

hcRED

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(F503/4A)-FLAG

Plasmid

#164974

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

F503A and F504A

Promoter

EF1a

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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Luciferase-pcw107-V5

Plasmid

#64649

Purpose

(control) when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

n/a

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

PGK

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
pLX317-VRK1

Plasmid

#199643

Purpose

Expresses VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

VRK1 (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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XE251 pCDNA3.1(zeo)-hDsh3

Plasmid

#16758

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3 (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Use

Tags

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Promoter

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-RpHLuorin2

Plasmid

#171722

Purpose

Synchronize trafficking of TNFa-RpHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

TNF (TNF Human)

Use

Tags

Streptavidin Binding Protein (SBP) and ratiometri…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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hCMV-IE1:RedF

Plasmid

#118064

Purpose

Transcriptional unit for constitutive expression of RedF in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

CMV:RedF:bGHF

Use

Luciferase and Synthetic Biology; Assembly of gb2…

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCl-HA-YAP

Plasmid

#27007

Depositor

Joan Massague

Article

Alarcon et al Cell. 2009 Nov 13. 139(4):757-69.

Insert

YAP (YAP1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2011

Availability

Academic Institutions and Nonprofits only
pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pXG290

Plasmid

#141284

Purpose

OE of HRI

Depositor

Martin Kampmann

Article

Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

Insert

HRI (EIF2AK1 Human)

Use

Tags

mClover

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-LATS2-KR

Plasmid

#33100

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

lats2 (Lats2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K655R, Kinase Dead

Promoter

CMV

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only