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Plasmid#115494PurposeExpression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cellsDepositorUseTagsiRFP, mCherry, and mKOExpressionMammalianMutationmCherry S227F, mCherry G229R, mKO V211GPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only
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pJPN0016
Plasmid#112077PurposetagRFP module CTDepositorInsertTagRFP
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pGTag
Plasmid#117818PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertTagRFP
UseSynthetic BiologyTagsCAAXExpressionMutationPromoterAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S2-RAB_EKARev
Plasmid#160726PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S2 protein scaffold.DepositorInsertS2-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S3-RAB_EKARev
Plasmid#160727PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter RAB-EKARev (ERK indicator), VSV-G tag, and S3 protein scaffold.DepositorInsertS3-RAB_EKARev
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-TagBFP
Plasmid#117786PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh1
Plasmid#168984PurposeExpresses RFP cDNA and SAE1 shRNA 1 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Control-cx43.4-48H-pPRISM
Plasmid#117801PurposeControl to test technique on a target that is known to workDepositorInsertTagRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-eGFP
Plasmid#117785PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000231725
Plasmid#78163PurposeshRFP controlDepositorInsertRFP
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLY164
Plasmid#184140PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 3)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R3, PrhaB, and PtetAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-gcry-TagBFP
Plasmid#117783PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UBC-S1-jRCaMP1a
Plasmid#160728PurposeSignaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.DepositorInsertS1-jRCaMP1a
UseTagsExpressionMammalianMutationN/APromoterHuman ubiquitin C (UBC) promoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPRISM-Fuse-cmlc2-eGFP
Plasmid#117788PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorInsertmRFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000072213
Plasmid#78159PurposeshRFP controlDepositorInsertRFP
UseRNAiTagsExpressionMutationPromoterhU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLY162
Plasmid#184138PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 1)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R1, PrhaB, and PtetAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY163
Plasmid#184141PurposeReporter circuit for hijacking RFP mRNA as CRISPR RNA (target site 2)DepositorInsertsdCas9
tetR
pspFΔHTH::λN22plus
sfgfp
rhaS
UseSynthetic BiologyTagsASV tag and λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPcon, PpspA-R2, PrhaB, and PtetAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-SAE1_sh3
Plasmid#168985PurposeExpresses RFP cDNA and SAE1 shRNA 3 in mammalian cellsDepositorInsertSUMO1 Activating Enzyme Subunit 1 (SAE1 Human)
UseTagsExpressionMammalianMutationPromoterUbc promoterAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJPN0015
Plasmid#112081PurposetagRFP module NT2DepositorInsertTagRFP
UseTagsExpressionPlantMutationBsaI and BpiI restriction sites have been remove…PromoterAvailable sinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pELlR1
Plasmid#172569PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLlacO-1Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only