Skip to main content
Addgene
Showing: 4921 - 4960 of 7539 results

  6. iEscSnFR

    Plasmid
    #182807
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iEscSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182807-plasmid-map-sequence-id-358170

    Enlarge

  7. iFluoxSnFR

    Plasmid
    #182808
    Purpose
    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists
    Depositor
    Henry Lester
    Article
    Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do
    Insert
    iFluoxSnFR
    Use
    Tags
    6xHis, HA and Myc
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    182808-plasmid-map-sequence-id-358173

    Enlarge

  10. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid
    #193764
    Purpose
    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)
    Depositor
    Tobias Meyer
    Article
    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do
    Insert
    Cdt1ΔCy-mCherry (CDT1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…
    Promoter
    TRE
    Available since
    Jan. 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    193764-plasmid-map-sequence-id-380225

    Enlarge

  11. pSN-A112C-CP8B1

    Plasmid
    #191240
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    Use
    Tags
    nuclease A from S. aureus fused to the poly-Leu T…
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine in SN, and predic…
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191240-plasmid-map-sequence-id-380472

    Enlarge

  26. pGEX-2T-CBP(1-287)

    Plasmid
    #182839
    Purpose
    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)
    Depositor
    Peter Harte
    Article
    Tie et al Development. 2014 Mar;141(5):1129-39. do
    Insert
    CBP1-287
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182839-plasmid-map-sequence-id-360063

    Enlarge

  34. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid
    #179160
    Purpose
    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.
    Depositor
    Masahito Ikawa
    Article
    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do
    Insert
    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    Use
    Tags
    6XHIS and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    179160-plasmid-map-sequence-id-352799

    Enlarge

  37. p35S_EVDm1

    Plasmid
    #167121
    Purpose
    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE (EVD) (AT5G17125 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    mutated four nucleotides at the snoRNA U1 binding…
    Promoter
    CaMV 35S
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    167121-plasmid-map-sequence-id-333657

    Enlarge

  38. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Depositor
    Olivier Voinnet, Arturo Marí-Ordóñez
    Article
    Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Use
    Tags
    mGFP on N-term, GUS on C-term
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    167123-plasmid-map-sequence-id-331746

    Enlarge

Showing: 4921 - 4960 of 7539 results